Affinity purified from pooled serum. Learn more.

Olig2 (Ser10,13,14) Antibody

Catalog #: p1540-1013 Categories: , , , Datasheet:

$119.00$380.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Mouse, Rat
Expected Reactivity:
Guinea Pig, Human, Non-human primate, Zebra fish
Applications:
WB 1:1000 Don't see your application?
Species:
Rabbit
Gene Name:
OLIG2
Molecular Weight:
~32 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1540-1013, RRID:AB_2492194
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser10,13,14 of Olig2.

The antibody is  prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho- peptide affinity columns.

Biological Significance: ExpandCollapse

Olig2 is a well conserved bHLH transcription factor that shows both anti-neural functions and pro-neural functions at different stages in the formation of the oligodendrocyte lineage (Sun et al., 2011). Olig2 is expressed in 100% of the human diffuse gliomas irrespective of grade and required for intracranial tumor formation in a genetically relevant model of malignant glioma (Ligon et al., 2004; Ligon et al., 2007). A developmentally regulated triple serine motif at positions 10, 13 and 14 in the amino terminus is well conserved across species ranging from humans to zebrafish and is essential for Olig2 proliferative function in both normal and malignant neural progenitors (Sun et al., 2011). All three serine residues must be mutated to achieve a strong loss-of-function or gain-of-function phenotype, suggesting that the phosphorylation state of Olig2 represents a significant conformational change in the amino terminus (Sun et al., 2011).

Synonyms: ExpandCollapse

• Basic domain helix loop helix protein class B 1 antibody
• Basic helix loop helix protein class B 1 antibody
• BHLHB antibody
• bHLHB1 antibody
• bHLHe19 antibody
• Class B basic helix loop helix protein 1 antibody
• Class B basic helix-loop-helix protein 1 antibody
• class E basic helix loop helix protein 19 antibody
• Class E basic helix-loop-helix protein 19 antibody
• Human protein kinase C binding protein RACK17 antibody
• Olig2 antibody
• OLIG2_HUMAN antibody
• Oligo2 antibody
• Oligodendrocyte lineage transcription factor 2 antibody
• Oligodendrocyte specific bHLH transcription factor 2 antibody
• Oligodendrocyte transcription factor 2 antibody
• OTTHUMP00000067569 antibody
• OTTHUMP00000067570 antibody
• PRKCBP2 antibody
• Protein kinase C binding protein 2 antibody
• Protein kinase C binding protein RACK17 antibody
• Protein kinase C-binding protein 2 antibody
• Protein kinase C-binding protein RACK17 antibody
• RACK17 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Ligon, K.L., Alberta, J.A., Kho, A.T., Weiss, J., Kwaan, M.R., Nutt, C.L., Louis, D.N., Stiles, C.D. and Rowitch, D.H., 2004. The oligodendroglial lineage marker OLIG2 is universally expressed in diffuse gliomas. Journal of neuropathology and experimental neurology, 63(5), pp.499-509. PMID: 15198128

Ligon, K.L., Huillard, E., Mehta, S., Kesari, S., Liu, H., Alberta, J.A., Bachoo, R.M., Kane, M., Louis, D.N., DePinho, R.A. and Anderson, D.J., 2007. Olig2-regulated lineage-restricted pathway controls replication competence in neural stem cells and malignant glioma. Neuron, 53(4), pp.503-517. PMID: 17296553

Sun, Y., Meijer, D.H., Alberta, J.A., Mehta, S., Kane, M.F., Tien, A.C., Fu, H., Petryniak, M.A., Potter, G.B., Liu, Z. and Powers, J.F., 2011. Phosphorylation state of Olig2 regulates proliferation of neural progenitors. Neuron, 69(5), pp.906-917. PMID: 21382551

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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