Rabbit polyclonal antibody
Pooled Serum- Formulation:
- Affinity Purified from Pooled Serum
- 100% Homology:
- Rat, Mouse, Human, Non-human primate, Zebra fish, Guinea Pig
- Applications:
- WB 1:1,000
- Species:
- Rabbit
- Gene Name:
- OLIG2
- Molecular Reference:
- ~32 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# p1540-1013, RRID:AB_2492194
The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser10,13,14 of Olig2.
The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho- peptide affinity columns.
100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.
Storage
100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.
General References
Ligon KL, Alberta JA, Kho AT, Weiss J, Kwaan MR, Nutt CL, Louis DN, Stiles CD, Rowitch DH. The oligodendroglial lineage marker OLIG2 is universally expressed in diffuse gliomas. J Neuropathol Exp Neurol. 2004;63:499–509.
Ligon KL, Huillard E, Mehta S, Kesari S, Liu H, Alberta JA, Bachoo RM, Kane M, Louis DN, Depinho RA, Anderson DJ, Stiles CD, Rowitch DH. (2007) Olig2-regulated lineage-restricted pathway controls replication competence in neural stem cells and malignant glioma. Neuron. 2007;53:503–17.
Sun Y, Meijer DH, Alberta JA, Mehta S, Kane MF, Tien AC, Fu H, Petryniak MA, Potter GB, Liu Z, Powers JF, Runquist IS, Rowitch DH, Stiles CD. (2011) Phosphorylation state of Olig2 regulates proliferation of neural progenitors. Neuron 69(5):906-17
