Olig2 Antibody

Catalog #: 1538-Olig2 Categories: , , Datasheet:

$109.00$365.00

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Perfect for IHC!

Formulation:
Protein A Purified
100% Homology:
Rat, Mouse, Human
Applications:
WB 1:3,000IHC 1:1,000IP 1:500-1:1,000
Species:
Rabbit polyclonal
Gene Name:
OLIG2
Molecular Reference:
~32 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1538-Olig2, RRID:AB_2492193
Antigen/Purification: ExpandCollapse

The antigen is a recombinant mouse Olig2.

The antibody is Protein A purified rabbit serum.

Biological Significance: ExpandCollapse

Olig2 is a transcription factor which is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Expressed in the ventral spinal cord as early as 9.5 dpc and scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. From 10.5 through 14.5 dpc, Olig2 is expressed in numerous cells in the ventricular and subventricular zones of the lateral and medial ganglionic eminences, suggesting that expression might not be limited to the oligodendrocytic lineage. Olig2 has further been shown to be critical in the proliferation of malignant glioma in brain tumors (Ligon et al., 2007)

Storage

Liquid in 100 mM Glycine, 200 mM Tris, pH 8.0. Contains no preservative.

For long term storage –20° C is recommended in undiluted aliquots. Stable at –20° C for for up to one year after date of receipt. Avoid freeze/thaw cycles.

Product Specific References

Kucharova, K. and Stallcup, W.B., 2017. Distinct NG2 proteoglycan-dependent roles of resident microglia and bone marrow-derived macrophages during myelin damage and repair. PloS one, 12(11), p.e0187530. PMID: 29095924

Li, W., Gorantla, S., Gendelman, H.E. and Poluektova, L.Y., 2017. Systemic HIV-1 infection produces a unique glial footprint in humanized mouse brains. Disease models & mechanisms, 10(12), pp.1489-1502. PMID: 29084769

Colón, J.M., González, P.A., Cajigas, Á., Maldonado, W.I., Torrado, A.I., Santiago, J.M., Salgado, I.K. and Miranda, J.D., 2018. Continuous tamoxifen delivery improves locomotor recovery 6 h after spinal cord injury by neuronal and glial mechanisms in male rats. Exp Neurol. 2018 Jan;299(Pt A):109-121. PMID: 29037533

Kucharova, K., & Stallcup, W. B. (2015). NG2-proteoglycan-dependent contributions of oligodendrocyte progenitors and myeloid cells to myelin damage and repair. Journal of neuroinflammation, 12(1), 161. PMID: 26338007

Mauney, S. A., Pietersen, C. Y., Sonntag, K. C., & Woo, T. U. W. (2015). Differentiation of oligodendrocyte precursors is impaired in the prefrontal cortex in schizophrenia. Schizophrenia Research, 169(1), 374-380. PMID: 26585218

Norwood J, Franklin JM, Sharma D, D’Mello SR (2014) Histone deacetylase 3 is necessary for proper brain development. J Biol Chem. 2014 Dec 12;289(50):34569-34582. PMID: 25339172

Sharma, V.P., Anderson, N.T. and Geusz, M.E., 2014. Circadian properties of cancer stem cells in glioma cell cultures and tumorspheres. Cancer letters, 345(1), pp.65-74. PMID: 24333739 

Olig2 Antibody
Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~32 kDa Olig2 protein.
Olig2 Antibody
Immunostaining of primary rat cortical neuroepithelial cells with anti-Olig2 (red). The cells were treated with basic FGF for 30 hours prior to staining (this induces Olig2 expression in these primary cells).

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