Olig2 Antibody

Catalog #: 1538-Olig2 Categories: , , Datasheet:

$109.00$365.00

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Perfect for IHC!

Formulation:
Protein A Purified
Species Tested:
Human, Mouse, Rat
Applications:
WB 1:3000IHC 1:200-1:1000IP 1:500-1:1,000ICC 1:1000
Species:
Rabbit polyclonal
Gene Name:
OLIG2
Molecular Weight:
~32 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1538-Olig2, RRID:AB_2492193
Antigen/Purification: ExpandCollapse

The antigen is a recombinant mouse Olig2.

The antibody is Protein A purified rabbit serum.

Biological Significance: ExpandCollapse

Olig2 is a transcription factor which is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Expressed in the ventral spinal cord as early as 9.5 dpc and scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. From 10.5 through 14.5 dpc, Olig2 is expressed in numerous cells in the ventricular and subventricular zones of the lateral and medial ganglionic eminences, suggesting that expression might not be limited to the oligodendrocytic lineage. Olig2 has further been shown to be critical in the proliferation of malignant glioma in brain tumors (Ligon et al., 2007)

Synonyms: ExpandCollapse

• Basic domain helix loop helix protein class B 1 antibody
• Basic helix loop helix protein class B 1 antibody
• BHLHB antibody
• bHLHB1 antibody
• bHLHe19 antibody
• Class B basic helix loop helix protein 1 antibody
• Class B basic helix-loop-helix protein 1 antibody
• class E basic helix loop helix protein 19 antibody
• Class E basic helix-loop-helix protein 19 antibody
• Human protein kinase C binding protein RACK17 antibody
• Olig2 antibody
• OLIG2_HUMAN antibody
• Oligo2 antibody
• Oligodendrocyte lineage transcription factor 2 antibody
• Oligodendrocyte specific bHLH transcription factor 2 antibody
• Oligodendrocyte transcription factor 2 antibody
• OTTHUMP00000067569 antibody
• OTTHUMP00000067570 antibody
• PRKCBP2 antibody
• Protein kinase C binding protein 2 antibody
• Protein kinase C binding protein RACK17 antibody
• Protein kinase C-binding protein 2 antibody
• Protein kinase C-binding protein RACK17 antibody
• RACK17 antibody

Storage

100 µl liquid in 100 mM Glycine, 200 mM Tris, pH 8.0. Contains no preservative. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Immunohistochemistry

Mouse brain and spinal cord

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunocytochemistry: Human| Rat
Immunohistochemistry: Human | Mouse
Western Blot: Human | Mouse | Rat


Immunocytochemistry: Human
PMID Dilution Publication
31091434 1:1000 Kim, H., et al. 2019. Pluripotent Stem Cell-derived Cerebral Organoids Reveal Human Oligodendrogenesis with Dorsal and Ventral Origins. Stem cell reports, 12(5), pp.890-905.

Immunocytochemistry: Rat
PMID Dilution Publication
24333739 not listed Sharma, V.P., et al. 2014. Circadian properties of cancer stem cells in glioma cell cultures and tumorspheres. Cancer letters, 345(1), pp.65-74.


Immunohistochemistry: Human
PMID Dilution Publication
29084769 1:500 Li, W., et al. 2017. Systemic HIV-1 infection produces a unique glial footprint in humanized mouse brains. Disease models & mechanisms, 10(12), pp.1489-1502.
26585218 1:1000 Mauney, S.A., et al. 2015. Differentiation of oligodendrocyte precursors is impaired in the prefrontal cortex in schizophrenia. Schizophrenia Research, 169(1), 374-380.

Immunohistochemistry: Mouse
PMID Dilution Publication
29095924 1:200 Kucharova, K., et al. 2017. Distinct NG2 proteoglycan-dependent roles of resident microglia and bone marrow-derived macrophages during myelin damage and repair. PloS one, 12(11), p.e0187530.
26338007 1:200 Kucharova, K., et al. 2015. NG2-proteoglycan-dependent contributions of oligodendrocyte progenitors and myeloid cells to myelin damage and repair. Journal of neuroinflammation, 12(1), 161.
25339172 1:500 Norwood, J., et al. 2014. Histone deacetylase 3 is necessary for proper brain development. J Biol Chem.,289(50):34569-34582.


Western Blot: Human
PMID Dilution Publication
31091434 1:2000 Kim, H., et al. 2019. Pluripotent Stem Cell-derived Cerebral Organoids Reveal Human Oligodendrogenesis with Dorsal and Ventral Origins. Stem cell reports, 12(5), pp.890-905.

Western Blot: Mouse
PMID Dilution Publication
25339172 1:500 Norwood, J., et al. 2014. Histone deacetylase 3 is necessary for proper brain development. J Biol Chem.,289(50):34569-34582.

Western Blot: Rat
PMID Dilution Publication
29037533 1:3000 Colón, J.M., et al. 2018. Continuous tamoxifen delivery improves locomotor recovery 6 h after spinal cord injury by neuronal and glial mechanisms in male rats. Exp Neurol. 2018 Jan;299(Pt A):109-121.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Olig2 Antibody
Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~32 kDa Olig2 protein.

Click here to view our Western blotting and lysate preparation protocols.
Olig2 Antibody
Immunostaining of primary rat cortical neuroepithelial cells with anti-Olig2 (cat # 1538-OLIG2, red, 1:1000). The cells were treated with basic FGF for 30 hours prior to staining (this induces Olig2 expression in these primary cells).
An illustration differentiating mouse and human oligodendrocytes in the process of glial humanization of a mouse brain model used to study HIV-1 infection. Immunoflourescence of paraffin embedded chimeric mouse brain 5-µm thick tissue section immunolabeling oligodendrocytes with anti-OLIG2 antibody (cat. 1538-OLIG2, 1:500, green) and a human specific anti-nuclei antibody(clone #3E1.3). The blue stain is DAPI. The anti-OLIG2 labels the oligodendrocytes of both human and mouse, where the human specific anti-nuclei antibody differentiates the mouse and human oligodendrocytes. The images were kindly provided by Larisa Poluektova, University of Nebraska Medical Center.

Learn more about this nuclear marker in this publication from Disease Models and Mechanisms.

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