Affinity purified from pooled serum. Learn more.

NSF Antibody

We are the original manufacturer of our rabbit polyclonal NSF antibody, affinity purified from pooled serum. It is excellent for use in WB.

Catalog #: 1525-NSF Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Rat
Expected Reactivity:
Human, Mouse
Applications:
WB 1:1000 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
NSF
Molecular Weight:
~75 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1525-NSF, RRID:AB_2492191
Antigen/Purification: ExpandCollapse

The antigen is a peptide corresponding to amino acid residues from the C-terminal region of rat NSF, conjugated to keyhole limpet hemocyanin (KLH).

The antibody is prepared from pooled rabbit serum by affinity purification via chromatography on an affinity column made with the C-terminal peptide used as antigen.

Biological Significance: ExpandCollapse

NSF (N-ethylmaleimide sensitive fusion protein) is a critical component of the SNARE (soluble NSF attachment protein receptors) protein complex that is involved in synaptic vesicle trafficking. Specifically, NSF has been found to be essential in membrane fusion. Furthermore, NSF has been recently demonstrated to bind other protein complexes such as AMPA receptor subunits (GluR2), GATE-16, LMA-1 and Rabs suggesting a more diverse role in the assembly of various protein complexes (Whiteheart et al., 2004).

Synonyms: ExpandCollapse

• N ethylmaleimide sensitive factor antibody
• N ethylmaleimide sensitive factor like protein antibody
• N ethylmaleimide sensitive fusion protein antibody
• N-ethylmaleimide-sensitive fusion protein antibody
• NEM sensitive fusion protein antibody
• NEM-sensitive fusion protein antibody
• NSF antibody
• NSF_HUMAN antibody
• SKD 2 antibody
• SKD2 antibody
• Vesicle fusing ATPase antibody
• Vesicle-fusing ATPase antibody
• Vesicular fusion protein NSF antibody
• Vesicular-fusion protein NSF antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Schweizer, F.E., Dresbach, T., DeBello, W.M., O’Connor, V., Augustine, G.J. and Betz, H., 1998. Regulation of neurotransmitter release kinetics by NSF. Science, 279(5354), pp.1203-1206. PMID: 9469810

Matveeva, E.A., Whiteheart, S.W., Vanaman, T.C. and Slevin, J.T., 2001. Phosphorylation of the N-ethylmaleimide-sensitive factor is associated with depolarization-dependent neurotransmitter release from synaptosomes. Journal of Biological Chemistry, 276(15), pp.12174-12181. PMID: 11278345

Whiteheart, S.W. and Matveeva, E.A., 2004. Multiple binding proteins suggest diverse functions for the N-ethylmaleimide sensitive factor. Journal of structural biology, 146(1-2), pp.32-43. PMID: 15037235

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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