Affinity purified from pooled serum. Learn more.

NSF Antibody

Catalog #: 1525-NSF Category: Datasheet:


Mouse monoclonal antibody

Pooled Serum
100 µl
Affinity Purified from Pooled Serum
Rat, Mouse, Human
WB 1:1,000
Molecular Reference:
~75 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1525-NSF, RRID:AB_2492191
Antigen/Purification: ExpandCollapse

The antigen is a peptide corresponding to amino acid residues from the C-terminal region of rat NSF, conjugated to keyhole limpet hemocyanin (KLH).

The antibody is prepared from pooled rabbit serum by affinity purification via chromatography on an affinity column made with the C-terminal peptide used as antigen.

Biological Significance: ExpandCollapse

NSF (N-ethylmaleimide sensitive fusion protein) is a critical component of the SNARE (soluble NSF attachment protein receptors) protein complex that is involved in synaptic vesicle trafficking. Specifically, NSF has been found to be essential in membrane fusion. Furthermore, NSF has been recently demonstrated to bind other protein complexes such as AMPA receptor subunits (GluR2), GATE-16, LMA-1 and Rabs suggesting a more diverse role in the assembly of various protein complexes (Whiteheart et al., 2004).


100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

General References

Klimek V, Stockmeier C, Overholser J, Melt zer HY, Kalka S, Dilley G, Ordway GA (1997) Reduced levels of norepinephrine transporters in the locus coeruleus in major depression. J. Neurosci. 17(21):8451-8458

Kim CH, Hahn MK, Joung Y, Anderson SL, Steele AH, Mazei-Robinson MS, Gizer I, Teicher MH, Cohen BM, Robertson D, Waldman ID, Blakely RD, Kim KS (2006) A Polymorphism in the norepinephrine transporter gene alters promoter activi ty and is associated with attention-deficit hyperactivity disorder. Proc Nat’l Acad Sci USA 103(50):19164-9

NSF Antibody
Western blot of rat striatal lysate showing specific labeling of the ~75 kDa NSF protein.

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