Affinity purified from pooled serum. Learn more.

NMDA Receptor NR2B Subunit (Tyr1472) Antibody

Catalog #: p1516-1472 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse, Rat
Species Reactivity:
Bovine, Chicken, Non-human primate, Zebra fish, Canine
Applications:
WB 1:1000
Species:
Rabbit
Gene Name:
GRIN2B
Molecular Weight:
~180 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1516-1472, RRID:AB_2492182
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr1472 of NMDA NR2B.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

The ion channels activated by glutamate that are sensitive to N-methyl-Daspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). Channels with physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Overexpression of the NR2B-subunit of the NMDA Receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression (Clayton et al., 2002a; Clayton et al., 2002b). Recent work suggests that phosphorylation of Tyr1472 on NR2B may regulate the functional expression the receptor in LTP and other forms of plasticity (Nakazawa et al., 2001; Roche et al., 2001).

Synonyms: ExpandCollapse

• AW490526 antibody
• EIEE27 antibody
• Glutamate [NMDA] receptor subunit epsilon 2 antibody
• Glutamate [NMDA] receptor subunit epsilon-2 antibody
• Glutamate Receptor Ionotropic N Methyl D Aspartate 2B antibody
• Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B antibody
• Glutamate receptor ionotropic NMDA2B antibody
• Glutamate receptor subunit epsilon 2 antibody
• Glutamate receptor, ionotropic, NMDA2B (epsilon 2) antibody
• GRIN 2B antibody
• GRIN2B antibody
• hNR 3 antibody
• hNR3 antibody
• MGC142178 antibody
• MGC142180 antibody
• MRD6 antibody
• N methyl D asparate receptor channel subunit epsilon 2 antibody
• N methyl D aspartate receptor subtype 2B antibody
• N methyl D aspartate receptor subunit 2B antibody
• N methyl D aspartate receptor subunit 3 antibody
• N-methyl D-aspartate receptor subtype 2B antibody
• N-methyl-D-aspartate receptor subunit 3 antibody
• NMDA NR2B antibody
• NMDA R2B antibody
• NMDAR2B antibody
• NMDE2 antibody
• NMDE2_HUMAN antibody
• NME2 antibody
• NR2B antibody
• NR3 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Human | Mouse | Rat


Western Blot: Mouse
PMID Dilution Publication
31135041 1:1000 Dedek, A., et al. 2019. Loss of STEP61 couples disinhibition to N-methyl-d-aspartate receptor potentiation in rodent and human spinal pain processing. Brain, 142(6), pp.1535-1546.

Western Blot: Mouse
PMID Dilution Publication
30551522 not listed Zhang, X., et al. 2019. Effects of 5-Aza on p-Y1472 NR2B related to learning and memory in the mouse hippocampus. Biomedicine & Pharmacotherapy, 109, pp.701-707.
27335408 not listed Xiao, X., et al. 2016. Disruption of Coordinated Presynaptic and Postsynaptic Maturation Underlies the Defects in Hippocampal Synapse Stability and Plasticity in Abl2/Arg-Deficient Mice. The Journal of Neuroscience, 36(25), 6778-6791.
27094400 1:1000 Zamzow, D.R., et al. 2016. Higher levels of phosphorylated Y1472 on GluN2B subunits in the frontal cortex of aged mice are associated with good spatial reference memory, but not cognitive flexibility. AGE, 38(3), 1-17.
22544749 1:1000 Xu, J., et al. 2012. Striatal-enriched protein-tyrosine phosphatase (STEP) regulates Pyk2 kinase activity. J Biol Chem. Jun 15;287(25):20942-56.
22523092 1:500 Gladding, C.M., et al. 2012. Calpain and Striatal-Enriched protein tyrosine phosphatase (STEP) activation contribute to extrasynaptic NMDA receptor localization in a Huntington’s disease mouse model. Hum Mol Genet. Sep 1;21(17):3739-52.
21464302 1:1000 Hinklin, T.R., et al. 2011. Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase. PNAS, Apr 2011; 108: 6650 – 6655.
20427654 1:1000 Kurup, P., et al. 2010. Aβ-mediated NMDA receptor endocytosis in Alzheimer’s disease involves ubiquitination of the tyrosine phosphatase STEP61. Journal of Neuroscience, 30(17), pp.5948-5957.

Western blot: Rat
PMID Dilution Publication
27127657 not listed Jang, S.S., et al. 2016. Seizure-Induced Regulations of Amyloid-β, STEP61, and STEP61 Substrates Involved in Hippocampal Synaptic Plasticity. Neural plasticity, 2016.
26391783 not listed Jang, S.S., et al. 2015. Regulation of STEP61 and tyrosine-phosphorylation of NMDA and AMPA receptors during homeostatic synaptic plasticity. Molecular brain, 8.
26277342 not listed Mao, L.M., et al. 2015. Dopaminergic and cholinergic regulation of Fyn tyrosine kinase phosphorylation in the rat striatum in vivo. Neuropharmacology, 99, 491-499.
24611998 1:1000 Chen, W., et al. 2014. BDNF released during neuropathic pain potentiates NMDA receptors in primary afferent terminals. European Journal of Neuroscience, 39(9), 1439-1454.
21464302 1:1000 Hinklin, T.R., et al. 2011. Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase. PNAS, Apr 2011; 108: 6650 – 6655.
21215796 1:1000 Castillo, C., et al. 2011. The N-methyl-D-aspartate-evoked cytoplasmic calcium increase in adult rat dorsal root ganglion neuronal somata was potentiated by substance P pretreatment in a protein kinase C-dependent manner. Neuroscience, 177, pp.308-320.
20427654 1:1000 Kurup, P., et al. 2010. Aβ-mediated NMDA receptor endocytosis in Alzheimer’s disease involves ubiquitination of the tyrosine phosphatase STEP61. Journal of Neuroscience, 30(17), pp.5948-5957.
19625523 1:1000 Xu, J., et al. 2009. Extrasynaptic NMDA receptors couple preferentially to excitotoxicity via calpain-mediated cleavage of STEP. Journal of Neuroscience, 29(29), pp.9330-9343.
12536146 1:1000 Alvested, R.M., et al. 2003. Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. Journal of Biological Chemistry, 278(13), pp.11020-11025.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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NMDA Receptor NR2B Subunit tyr1472 Antibody
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR phosphorylated at Tyr1472 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (400 units/100uL lysate for 30 min).

Click here to view our Western blotting and lysate preparation protocols.

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