Affinity purified from pooled serum. Learn more.

NMDA Receptor NR2B Subunit (Tyr1472) Antibody

We are the original manufacturer of our NMDAR NR2B (Tyr1472) rabbit polyclonal phosphospecific antibody, affinity purified from pooled serum. Optimized in WB.

Catalog #: p1516-1472 Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse, Rat
Expected Reactivity:
Bovine, Chicken, Non-human primate, Zebra fish, Canine
Applications:
WB 1:1000ICC 1:100 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
GRIN2B
Molecular Weight:
~180 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1516-1472, RRID:AB_2492182
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr1472 of NMDA NR2B.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

The ion channels activated by glutamate that are sensitive to N-methyl-Daspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). Channels with physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Overexpression of the NR2B-subunit of the NMDA Receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression (Clayton et al., 2002a; Clayton et al., 2002b). Recent work suggests that phosphorylation of Tyr1472 on NR2B may regulate the functional expression the receptor in LTP and other forms of plasticity (Nakazawa et al., 2001; Roche et al., 2001).

Synonyms: ExpandCollapse

• AW490526 antibody
• EIEE27 antibody
• Glutamate [NMDA] receptor subunit epsilon 2 antibody
• Glutamate [NMDA] receptor subunit epsilon-2 antibody
• Glutamate Receptor Ionotropic N Methyl D Aspartate 2B antibody
• Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B antibody
• Glutamate receptor ionotropic NMDA2B antibody
• Glutamate receptor subunit epsilon 2 antibody
• Glutamate receptor, ionotropic, NMDA2B (epsilon 2) antibody
• GRIN 2B antibody
• GRIN2B antibody
• hNR 3 antibody
• hNR3 antibody
• MGC142178 antibody
• MGC142180 antibody
• MRD6 antibody
• N methyl D asparate receptor channel subunit epsilon 2 antibody
• N methyl D aspartate receptor subtype 2B antibody
• N methyl D aspartate receptor subunit 2B antibody
• N methyl D aspartate receptor subunit 3 antibody
• N-methyl D-aspartate receptor subtype 2B antibody
• N-methyl-D-aspartate receptor subunit 3 antibody
• NMDA NR2B antibody
• NMDA R2B antibody
• NMDAR2B antibody
• NMDE2 antibody
• NMDE2_HUMAN antibody
• NME2 antibody
• NR2B antibody
• NR3 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunocytochemistry: Rat
Western Blot: Human | Mouse | Rat

Immunocytochemistry: Rat
PMIDDilutionPublication
326517561:100 Akkuratov, E.E., et al. 2020 Ouabain Modulates the Functional Interaction Between Na, K-ATPase and NMDA Receptor. Molecular Neurobiology, pp.1-13.

Western Blot: Mouse
PMIDDilutionPublication
311350411:1000Dedek, A., et al. 2019. Loss of STEP61 couples disinhibition to N-methyl-d-aspartate receptor potentiation in rodent and human spinal pain processing. Brain, 142(6), pp.1535-1546.

Western Blot: Mouse
PMIDDilutionPublication
315435051:1000Ajit, D., et al. 2020. A unique tau conformation generated by an acetylation-mimic substitution modulates P301S-dependent tau pathology and hyperphosphorylation. Journal of Biological Chemistry, 294(45), pp.16698-16711.
314522421:1000Liu, G., et al. 2019. Loss of tau and Fyn reduces compensatory effects of MAP2 for tau and reveals a Fyn‐independent effect of tau on calcium. Journal of neuroscience research. Aug 26.
30551522not listedZhang, X., et al. 2019. Effects of 5-Aza on p-Y1472 NR2B related to learning and memory in the mouse hippocampus. Biomedicine & Pharmacotherapy, 109, pp.701-707.
27335408not listedXiao, X., et al. 2016. Disruption of Coordinated Presynaptic and Postsynaptic Maturation Underlies the Defects in Hippocampal Synapse Stability and Plasticity in Abl2/Arg-Deficient Mice. The Journal of Neuroscience, 36(25), 6778-6791.
270944001:1000Zamzow, D.R., et al. 2016. Higher levels of phosphorylated Y1472 on GluN2B subunits in the frontal cortex of aged mice are associated with good spatial reference memory, but not cognitive flexibility. AGE, 38(3), 1-17.
225447491:1000Xu, J., et al. 2012. Striatal-enriched protein-tyrosine phosphatase (STEP) regulates Pyk2 kinase activity. J Biol Chem. Jun 15;287(25):20942-56.
225230921:500Gladding, C.M., et al. 2012. Calpain and Striatal-Enriched protein tyrosine phosphatase (STEP) activation contribute to extrasynaptic NMDA receptor localization in a Huntington’s disease mouse model. Hum Mol Genet. Sep 1;21(17):3739-52.
214643021:1000Hinklin, T.R., et al. 2011. Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase. PNAS, Apr 2011; 108: 6650 – 6655.
204276541:1000Kurup, P., et al. 2010. Aβ-mediated NMDA receptor endocytosis in Alzheimer’s disease involves ubiquitination of the tyrosine phosphatase STEP61. Journal of Neuroscience, 30(17), pp.5948-5957.

Western blot: Rat
PMIDDilutionPublication
324378891:500Zhu, D., et al. 2020. Angiotensin (1-7) through modulation of the NMDAR–nNOS–NO pathway and serotonergic metabolism exerts an anxiolytic-like effect in rats. Behavioral Brain Research, 390, p.112671.
27127657not listedJang, S.S., et al. 2016. Seizure-Induced Regulations of Amyloid-β, STEP61, and STEP61 Substrates Involved in Hippocampal Synaptic Plasticity. Neural plasticity, 2016.
26391783not listedJang, S.S., et al. 2015. Regulation of STEP61 and tyrosine-phosphorylation of NMDA and AMPA receptors during homeostatic synaptic plasticity. Molecular brain, 8.
26277342not listedMao, L.M., et al. 2015. Dopaminergic and cholinergic regulation of Fyn tyrosine kinase phosphorylation in the rat striatum in vivo. Neuropharmacology, 99, 491-499.
246119981:1000Chen, W., et al. 2014. BDNF released during neuropathic pain potentiates NMDA receptors in primary afferent terminals. European Journal of Neuroscience, 39(9), 1439-1454.
214643021:1000Hinklin, T.R., et al. 2011. Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase. PNAS, Apr 2011; 108: 6650 – 6655.
212157961:1000Castillo, C., et al. 2011. The N-methyl-D-aspartate-evoked cytoplasmic calcium increase in adult rat dorsal root ganglion neuronal somata was potentiated by substance P pretreatment in a protein kinase C-dependent manner. Neuroscience, 177, pp.308-320.
204276541:1000Kurup, P., et al. 2010. Aβ-mediated NMDA receptor endocytosis in Alzheimer’s disease involves ubiquitination of the tyrosine phosphatase STEP61. Journal of Neuroscience, 30(17), pp.5948-5957.
196255231:1000Xu, J., et al. 2009. Extrasynaptic NMDA receptors couple preferentially to excitotoxicity via calpain-mediated cleavage of STEP. Journal of Neuroscience, 29(29), pp.9330-9343.
125361461:1000Alvested, R.M., et al. 2003. Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. Journal of Biological Chemistry, 278(13), pp.11020-11025.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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