Affinity purified from pooled serum. Learn more.

NMDA Receptor NR2B Subunit (Tyr1472) Antibody

Catalog #: p1516-1472 Category: Datasheet:

$345.00

Rabbit polyclonal antibody

Pooled Serum
Size:
100 µl
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Rat, Mouse, Human, Bovine, Chicken, Non-human primate, Zebra fish, Canine
Applications:
WB 1:1,000
Species:
Rabbit
Gene Name:
GRIN2B
Molecular Reference:
~180 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1516-1472, RRID:AB_2492182
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr1472 of NMDA NR2B.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

The ion channels activated by glutamate that are sensitive to N-methyl-Daspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). Channels with physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Overexpression of the NR2B-subunit of the NMDA Receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression (Clayton et al., 2002a; Clayton et al., 2002b). Recent work suggests that phosphorylation of Tyr1472 on NR2B may regulate the functional expression the receptor in LTP and other forms of plasticity (Nakazawa et al., 2001; Roche et al., 2001).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Jang, S. S., Royston, S. E., Lee, G., Wang, S., & Chung, H. J. (2016). Seizure-Induced Regulations of Amyloid-β, STEP61, and STEP61 Substrates Involved in Hippocampal Synaptic Plasticity. Neural plasticity, 2016.  PMID: 27127657

Xiao, X., Levy, A. D., Rosenberg, B. J., Higley, M. J., & Koleske, A. J. (2016). Disruption of Coordinated Presynaptic and Postsynaptic Maturation Underlies the Defects in Hippocampal Synapse Stability and Plasticity in Abl2/Arg-Deficient Mice. The Journal of Neuroscience, 36(25), 6778-6791.  PMID: 27335408

Zamzow, D. R., Elias, V., Acosta, V. A., Escobedo, E., & Magnusson, K. R. (2016). Higher levels of phosphorylated Y1472 on GluN2B subunits in the frontal cortex of aged mice are associated with good spatial reference memory, but not cognitive flexibility. AGE, 38(3), 1-17. PMID: 27094400

Jang, S. S., Royston, S. E., Xu, J., Cavaretta, J. P., Vest, M. O., Lee, K. Y., Lee, S., Jeong, H.G., Lombroso, P., & Chung, H. J. (2015). Regulation of STEP61 and tyrosine-phosphorylation of NMDA and AMPA receptors during homeostatic synaptic plasticity. Molecular brain, 8. PMID: 26391783

Mao, L. M., & Wang, J. Q. (2015). Dopaminergic and cholinergic regulation of Fyn tyrosine kinase phosphorylation in the rat striatum in vivo. Neuropharmacology, 99, 491-499. PMID: 26277342

Chen, W., Walwyn, W., Ennes, H. S., Kim, H., McRoberts, J. A., & Marvizón, J. C. G. (2014). BDNF released during neuropathic pain potentiates NMDA receptors in primary afferent terminals. European Journal of Neuroscience, 39(9), 1439-1454. PMID: 24611998

Knox, R., Brennan-Minnella, A. M., Lu, F., Yang, D., Nakazawa, T., Yamamoto, T., Swanson, R.A., Ferriero, D.M., & Jiang, X. (2014). NR2B phosphorylation at tyrosine 1472 contributes to brain injury in a rodent model of neonatal hypoxia-ischemia. Stroke, 45(10), 3040-3047. PMID: 25158771

Gladding CM, Sepers MD, Xu J, Zhang LY, Milnerwood AJ, Lombroso PJ, Raymond LA (2012) Calpain and Striatal-Enriched protein tyrosine phosphatase (STEP) activation contribute to extrasynaptic NMDA receptor localization in a Huntington’s disease mouse model. Hum Mol Genet. Sep 1;21(17):3739-52. PMID: 22523092

Xu J, Kurup P, Bartos JA, Patriarchi T, Hell JW, Lombroso PJ (2012) Striatal-enriched protein-tyrosine phosphatase (STEP) regulates Pyk2 kinase activity. J Biol Chem Jun 15;287(25):20942-56. PMID: 22544749

Tianna R. Hicklin, Peter H. Wu, Richard A. Radcliffe, Ronald K. Freund, Susan M. Goebel-Goody, Paulo R. Correa, William R. Proctor, Paul J. Lombroso, and Michael D. Browning (2011) Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase PNAS, Apr 2011; 108: 6650 – 6655. PMID: 21464302

NMDA Receptor NR2B Subunit tyr1472 Antibody
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR phosphorylated at Tyr1472 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (400 units/100uL lysate for 30 min).

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