Excellent neuronal marker for use in IHC!
- Affinity Purified from Pooled Serum
- 100% Homology:
- Rat, Mouse, Human
- WB 1:1,000IHC 1:1,000 to 1:2,000IP 3 µl per 200 µg lysate
- Rabbit polyclonal
- Gene Name:
- Molecular Reference:
- ~180 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# 1496-NR2B, RRID:AB_2492176
The antigen is a fusion protein from the C-terminus of the NR2B subunit of rat NMDA receptor.
The antibody is prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.
The ion channels activated by glutamate that are sensitive to N-methyl-Daspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned and it can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. Overexpression of the NR2B-subunit of the NMDA receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression (Clayton et al., 2002a; Clayton et al., 2002b). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999).
100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.
Product Specific References
O’Leary, H., Bernard, P. B., Castano, A. M., & Benke, T. A. (2016). Enhanced long term potentiation and decreased AMPA receptor desensitization in the acute period following a single kainate induced early life seizure. Neurobiology of disease, 87, 134-144. PMID: 26706598
Bostrom, C. A., Majaess, N. M., Morch, K., White, E., & Christie, B. R. (2013). Rescue of NMDAR-dependent synaptic plasticity in Fmr1 knock-out mice. Cerebral cortex, bht237. PMID: 23968838
Megan L. Brady, Marvin R. Diaz, Anthony Iuso, Julie C. Everett, C. Fernando Valenzuela, and Kevin K. Caldwell. (2013) Moderate prenatal alcohol exposure reduces plasticity and alters NMDA receptor subunit composition in the dentate gyrus. J Neourosci. 33(3):1062-7. PMID: 23325244
Tianna R. Hicklin, Peter H. Wu, Richard A. Radcliffe, Ronald K. Freund, Susan M. Goebel-Goody, Paulo R. Correa, William R. Proctor, Paul J. Lombroso, and Michael D. Browning (2011) Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase PNAS, Apr 2011; 108: 6650 – 6655. PMID: 21464302
Pradeep Kurup, Yongfang Zhang, Jian Xu, Deepa V. Venkitaramani, Vahram Haroutunian, Paul Greengard, Angus C. Nairn, and Paul J. Lombroso (2010) A-beta-Mediated NMDA Receptor Endocytosis in Alzheimer’s Disease Involves Ubiquitination of the Tyrosine Phosphatase STEP61. J. Neurosci., 30: 5948 – 5957. PMID: 20427654
Jian Xu, Pradeep Kurup, Yongfang Zhang, Susan M. Goebel-Goody, Peter H. Wu, Ammar H. Hawasli, Matthew L. Baum, James A. Bibb, and Paul J. Lombroso Extrasynaptic NMDA Receptors Couple Preferentially to Excitotoxicity via Calpain-Mediated Cleavage of STEP (2009) J. Neurosci., 29: 9330 – 9343. PMID: 19625523
Kurtis D. Davies, Susan M. Goebel-Goody, Steven J. Coultrap, and Michael D. Browning (2008) Long Term Synaptic Depression That Is Associated with GluR1 Dephosphorylation but Not alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptor Internalization J. Biol. Chem., 283: 33138 – 33146. PMID: 18819923
Erin H. Norris and Sidney Strickland (2007) Modulation of NR2B-regulated contextual fear in the hippocampus by the tissue plasminogen activator system. PNAS, 104: 13473 - 13478. PMID: 17673549
Tatyana Chernova, Joern R. Steinert, Christopher J. Guerin, Pierluigi Nicotera, Ian D. Forsythe, and Andrew G. Smith (2007) Neurite Degeneration Induced by Heme Deficiency Mediated via Inhibition of NMDA Receptor-Dependent Extracellular Signal-Regulated Kinase 1/2 Activation J. Neurosci., 27: 8475 – 8485. PMID: 17687025