Affinity purified from pooled serum. Learn more.

NMDA Receptor NR2B Subunit Antibody, 50 µl

We are the original manufacturer of our NMDAR NR2B rabbit polyclonal antibody, formulated as neat serum. Optimized in WB, IHC, and IP.

Catalog #: 1498-NR2B Categories: , Datasheet:

$325.00

Pooled Serum
Size:
50 µl
Formulation:
Sera pooled, neat serum
Species Tested:
Rat, Mouse, Human
Applications:
WB 1:1000ICC 1:100 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
GRIN2B
Molecular Weight:
~180 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1498-NR2B, RRID:AB_2492177
Antigen/Purification: ExpandCollapse

The antigen is a fusion protein from the C-terminus region of the NR2B subunit of rat NMDA receptor.

The antibody is pooled, unpurified neat serum.

Biological Significance: ExpandCollapse

The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.

Synonyms: ExpandCollapse

• AW490526 antibody
• EIEE27 antibody
• Glutamate [NMDA] receptor subunit epsilon 2 antibody
• Glutamate [NMDA] receptor subunit epsilon-2 antibody
• Glutamate Receptor Ionotropic N Methyl D Aspartate 2B antibody
• Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B antibody
• Glutamate receptor ionotropic NMDA2B antibody
• Glutamate receptor subunit epsilon 2 antibody
• Glutamate receptor, ionotropic, NMDA2B (epsilon 2) antibody
• GRIN 2B antibody
• GRIN2B antibody
• hNR 3 antibody
• hNR3 antibody
• MGC142178 antibody
• MGC142180 antibody
• MRD6 antibody
• N methyl D asparate receptor channel subunit epsilon 2 antibody
• N methyl D aspartate receptor subtype 2B antibody
• N methyl D aspartate receptor subunit 2B antibody
• N methyl D aspartate receptor subunit 3 antibody
• N-methyl D-aspartate receptor subtype 2B antibody
• N-methyl-D-aspartate receptor subunit 3 antibody
• NMDA NR2B antibody
• NMDA R2B antibody
• NMDAR2B antibody
• NMDE2 antibody
• NMDE2_HUMAN antibody
• NME2 antibody
• NR2B antibody
• NR3 antibody

Storage

50 µl neat serum.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunocytochemistry: Human
Western Blot: Human | Mouse


Immunocytochemistry: Human
PMIDDilutionPublication
291296811:100 Schwab, A.J., et al. 2017 Decreased Sirtuin Deacetylase Activity in LRRK2 G2019S iPSC-Derived Dopaminergic Neurons. Stem Cell Reports, Vol 9, 1-14, Dec 12.


Western Blot: Human
PMIDDilutionPublication
29129681not listed Schwab, A.J., et al. 2017 Decreased Sirtuin Deacetylase Activity in LRRK2 G2019S iPSC-Derived Dopaminergic Neurons. Stem Cell Reports, Vol 9, 1-14, Dec 12.


Western Blot: Mouse
PMIDDilutionPublication
259719711:1000 Priya, A., et al. 2013 Specificity protein 4 functionally regulates the transcription of NMDA receptor subunits GluN1, GluN2A, and GluN2B. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1833(12), 2745-2756.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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