Affinity purified from pooled serum. Learn more.

NMDA Receptor NR2B Subunit Antibody, 10 µg

Catalog #: 1499-NR2B Categories: , , , Datasheet:

$365.00

Glutamatergic neuronal marker for IHC!

 

Pooled Serum
Size:
10 µg
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse, Rat
Applications:
WB 1:1000IHC 1:1000IP 3 µl per 200 µg lysate Don't see your application?
Species:
Rabbit polyclonal
Gene Name:
GRIN2B
Molecular Weight:
~180 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1499-NR2B, RRID:AB_2492175
Antigen/Purification: ExpandCollapse

The antigen is a fusion protein from the C-terminus of the NR2B subunit of rat NMDA receptor.

The antibody is prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Biological Significance: ExpandCollapse

The ion channels activated by glutamate that are sensitive to N-methyl-Daspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned and it can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. Overexpression of the NR2B-subunit of the NMDA receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression (Clayton et al., 2002a; Clayton et al., 2002b). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999).

Synonyms: ExpandCollapse

• AW490526 antibody
• EIEE27 antibody
• Glutamate [NMDA] receptor subunit epsilon 2 antibody
• Glutamate [NMDA] receptor subunit epsilon-2 antibody
• Glutamate Receptor Ionotropic N Methyl D Aspartate 2B antibody
• Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B antibody
• Glutamate receptor ionotropic NMDA2B antibody
• Glutamate receptor subunit epsilon 2 antibody
• Glutamate receptor, ionotropic, NMDA2B (epsilon 2) antibody
• GRIN 2B antibody
• GRIN2B antibody
• hNR 3 antibody
• hNR3 antibody
• MGC142178 antibody
• MGC142180 antibody
• MRD6 antibody
• N methyl D asparate receptor channel subunit epsilon 2 antibody
• N methyl D aspartate receptor subtype 2B antibody
• N methyl D aspartate receptor subunit 2B antibody
• N methyl D aspartate receptor subunit 3 antibody
• N-methyl D-aspartate receptor subtype 2B antibody
• N-methyl-D-aspartate receptor subunit 3 antibody
• NMDA NR2B antibody
• NMDA R2B antibody
• NMDAR2B antibody
• NMDE2 antibody
• NMDE2_HUMAN antibody
• NME2 antibody
• NR2B antibody
• NR3 antibody

Storage

10 µg antibody vial; lyophilized from 5 mM ammonium bicarbonate. Adequate amount of material to conduct 10-mini Western Blots.

The antibody should be reconstituted in 50 µl phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution it is recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunoprecipitation: Mouse | Rat
Western Blot: Mouse | Rat


Immunoprecipitation: Mouse
PMID Dilution Publication
17673549 not listed Chernova, T., et al. 2007 Neurite Degeneration Induced by Heme Deficiency Mediated via Inhibition of NMDA Receptor-Dependent Extracellular Signal-Regulated Kinase 1/2 Activation. J. Neurosci., 27: 8475 – 8485.


Immunoprecipitation: Rat
PMID Dilution Publication
12536146 3ul per 200ug lysate Alvestad, R.M., et al. 2003 Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. J Biol Chem 278:11020-11025.


Western Blot: Mouse
PMID Dilution Publication
25971971 not listed Tassin, T.C., et al. 2015 Regulation of ERK kinase by MEK1 kinase inhibition in the brain. Journal of Biological Chemistry, 290(26), pp.16319-16329.
23968838 1:2500 Bostrom, C.A., et al. 2013 Rescue of NMDAR-dependent synaptic plasticity in Fmr1 knock-out mice. Cerebral cortex, bht237.
23325244 1:500-1:1000 Brady, M.L., et al. 2013 Moderate prenatal alcohol exposure reduces plasticity and alters NMDA receptor subunit composition in the dentate gyrus. Journal of Neuroscience, 33(3), pp.1062-1067.
21464302 1:1000 Hicklin, T.R., et al. 2011 Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase. Proc Natl Acad Sci USA 108(16):6650-5.
17687025 not listed Chernova, T., et al. 2007 Neurite Degeneration Induced by Heme Deficiency Mediated via Inhibition of NMDA Receptor-Dependent Extracellular Signal-Regulated Kinase 1/2 Activation. J. Neurosci., 27: 8475 – 8485.
17673549 not listed Norris, E.H., et al. 2007 Modulation of NR2B-regulated contextual fear in the hippocampus by the tissue plasminogen activator system. PNAS, 104: 13473 – 1347.


Western Blot: Rat
PMID Dilution Publication
26706598 not listed O’Leary, H., et al. 2016 Enhanced long term potentiation and decreased AMPA receptor desensitization in the acute period following a single kainate induced early life seizure. Neurobiology of disease, 87, 134-144.
21464302 1:1000 Hicklin, T.R., et al. 2011 Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase. Proc Natl Acad Sci USA 108(16):6650-5.
18819923 1:1000 Davies, K.D., et al. 2008 Long Term Synaptic Depression That Is Associated with GluR1 Dephosphorylation but Not -Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptor Internalization. J. Biol. Chem., 283: 33138 – 33146.
12536146 1:1000 Alvestad, R.M., et al. 2003 Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. J Biol Chem 278:11020-11025.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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NMDA Receptor NR2B Subunit Antibody
Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDA receptor.

Click here to view our Western blotting and lysate preparation protocols.

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