Affinity purified from pooled serum. Learn more.

NMDA Receptor NR2A Subunit (Tyr1325) Antibody

We are the original manufacturer of our NMDAR NR2A (Tyr1325) rabbit polyclonal phosphospecific antibody, affinity purified from pooled serum. Optimized in WB.

Catalog #: p1514-1325 Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse, Rat
Expected Reactivity:
Bovine, Canine, Hamster, Sheep
Applications:
WB 1:1000 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
GRIN2A
Molecular Weight:
~180 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1514-1325, RRID:AB_2492174
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr1325 of rat NMDA NR2A.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). Channels with physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Recently, phosphorylation of Tyrosine 1325 of the NR2A subunit has been shown to be increased in human brain tissue sections from HIV-infected individuals with encephalitis (King et al., 2010). In addition, Tyr1325 phosphorylation has been linked with depression-related behavior (Taniguchi et al., 2009).

Synonyms: ExpandCollapse

• EPND antibody
• FESD antibody
• GluN2A antibody
• Glutamate [NMDA] receptor subunit epsilon-1 antibody
• Glutamate receptor antibody
• Glutamate receptor ionotropic N methyl D aspartate 2A antibody
• GRIN 2A antibody
• GRIN2A antibody
• hNR2A antibody
• LKS antibody
• N methyl D aspartate receptor channel, subunit epsilon 1 antibody
• N Methyl D Aspartate Receptor Subtype 2A antibody
• N methyl D aspartate receptor subunit 2A antibody
• N-methyl D-aspartate receptor subtype 2A antibody
• NMDA receptor subtype 2A antibody
• NMDAR 2A antibody
• NMDAR2A antibody
• NMDE1_HUMAN antibody
• NR2A antibody
• OTTHUMP00000160135 antibody
• OTTHUMP00000174531 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

 

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Human | Rat


Western Blot: Human
PMIDDilutionPublication
311350411:1000Dedek, A., et al. 2019. Loss of STEP61 couples disinhibition to N-methyl-d-aspartate receptor potentiation in rodent and human spinal pain processing. Brain, 142(6), pp.1535-1546.


Western blot: Rat
PMIDDilutionPublication
318357461:200Somkuwar, S.S., et al. 2019. Individual Differences in Ethanol Drinking and Seeking Behaviors in Rats Exposed to Chronic Intermittent Ethanol Vapor Exposure is Associated with Altered CaMKII Autophosphorylation in the Nucleus Accumbens Shell. Brain Sciences, 9(12), p.367.
311350411:1000Dedek, A., et al. 2019. Loss of STEP61 couples disinhibition to N-methyl-d-aspartate receptor potentiation in rodent and human spinal pain processing. Brain, 142(6), pp.1535-1546.
294414051:200Takashima, Y., et al. 2018. Neuroadaptations in the dentate gyrus following contextual cued reinstatement of methamphetamine seeking. Brain Structure and Function, pp.1-15.
262201711:200Staples, M.C., et al. 2015. Developmental effects of wheel running on hippocampal glutamate receptor expression in young and mature adult rats. Neuroscience. Jul 25. pii: S0306-4522(15)00672-7.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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