Our Anti-NMDA NR2A Subunit, N-terminus rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat NMDA NR2A Subunit, N-terminus and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.
Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2A subunit.
The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
ICC, WB
Rabbit
GRIN2A
180 kDa
Synthetic peptide corresponding to amino acid residues from the N-terminal region of the NR2A subunit of rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH).
Mouse, Rat
Bovine, Canine
AB_2492173
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
ICC: 1:500
Unconjugated
Specific for endogenous levels of the ~180 kDa NR2A subunit of the NMDA receptor. No reactivity towards the NR2B and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Alvestad RM, Grosshans DR, Coultrap SJ, Nakazawa T, Yamamoto T, Browning MD** (2003) Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. Journal of Biological Chemistry 278:11020-11025. PMID: 12536146
Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends in Neuroscience25:571-577. PMID: 12392932
Grosshans DR, Clayton DA, Coultrap SJ, Browning MD** (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nature Neuroscience 5:27-33. PMID: 11740502
Lovinger DM, White G, Weight FF (1989) Ethanol inhibits NMDA-activated ion current in hippocampal neurons. Science 243:1721-1724. PMID: 2467382
Lu W-Y, Xiong Z-G, Lei S, Orser BA, Browning MD**, MacDonald JF (1999) G-protein coupled receptors act via protein kinase C and Src to regulate NMDA receptors. Nature Neuroscience 2:331-338. PMID: 10204539
Snell LD, Nunley KR, Lickteig RL, Browning MD**, Tabakoff B, Hoffman PL (1996) Regional and subunit specific changes in NMDA receptor mRNA and immunoreactivity in mouse brain following chronic ethanol ingestion. Molecular Brain Research 40:71-78. PMID: 8840015
Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS (2003) Trafficking of NMDA receptors. Annual Reviews of Pharmacology and Toxicology 43:335-358. PMID: 12540744
**Dr. Michael Browning, a co-author of the above cited papers, is CEO and founder of PhosphoSolutions.
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