Affinity purified from pooled serum. Learn more.

NMDA Receptor NR1 Subunit, Splice Variant N1 Antibody

We are the original manufacturer of our NMDAR NR1 N1 splice variant rabbit polyclonal antibody, affinity purified from pooled serum. Optimized in WB and IHC.

Catalog #: 1504-N1 Categories: , Datasheet:

$380.00

Pooled Serum
Size:
25 µg
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Mouse, Rat
Expected Reactivity:
Canine, Chicken, Human, Non-human primate, Xenopus
Applications:
WB 1:1000IHC 1:1000 to 1:2000 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
GRIN1
Molecular Weight:
~120 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1504-N1, RRID:AB_2492169
Antigen/Purification: ExpandCollapse

The antigen is a peptide from the NR1 subunit, N1 splice variant insert of rat NMDA receptor.

The antibody is prepared from pooled rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Biological Significance: ExpandCollapse

The ion channels activated by glutamate that are sensitive to N-methyl-Daspartate (NMDA) are designated NMDA Receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). There are a number of different splice variants of the NR1-Subunit (Foldes et al., 1994; Zukin and Bennett, 1995). Differential splicing of three exons in the NR1-Subunit generates up to eight NR1-Subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2′). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.

Synonyms: ExpandCollapse

• GluN1 antibody
• Glutamate [NMDA] receptor subunit zeta-1 antibody
• Glutamate receptor ionotropic N methyl D aspartate 1 antibody
• Glutamate receptor ionotropic, N-methyl-D aspartate, subunit 1 antibody
• glutamate receptor ionotropic, NMDA 1 antibody
• Grin1 antibody
• MRD8 antibody
• N methyl D aspartate receptor antibody
• N methyl D aspartate receptor channel subunit zeta 1 antibody
• N methyl D aspartate receptor subunit NR1 antibody
• N-methyl-D-aspartate receptor subunit NR1 antibody
• NMD-R1 antibody
• NMDA 1 antibody
• NMDA R1 antibody
• NMDAR1 antibody
• NMDA receptor 1 antibody
• NMDA1 antibody
• NMDAR antibody
• NMDZ1_HUMAN antibody
• NR1 antibody

Storage

25 µg antibody vial; lyophilized from 5 mM ammonium bicarbonate. Adequate amount of material to conduct 10-mini Western Blots.

The antibody should be reconstituted in 50 µl phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution it is recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Mouse


Western Blot: Mouse
PMIDDilutionPublication
233252441:500 Brady, M.L., et al. 2013 Moderate prenatal alcohol exposure reduces plasticity and alters NMDA receptor subunit composition in the dentate gyrus. Journal of Neuroscience. 33(3):1062-7.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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