Affinity purified from pooled serum. Learn more.

Nicotinic Acetylcholine Receptor (nAChR) β4 Antibody

We are the original manufacturer of our nAChR β4 rabbit polyclonal antibody, affinity purified from pooled serum. Optimized in WB.

Catalog #: 1471-nACB4 Categories: , Datasheet:


  • SizePrice
Pooled Serum
Affinity Purified from Pooled Serum
Expected Reactivity:
Mouse, Rat
WB 1:1000 Don't see your application?
Host Species:
Rabbit Polyclonal
Molecular Weight:
~52 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1471-nACB4, RRID:AB_2619714
Antigen/Purification: ExpandCollapse

The antigen is a fusion protein from the cytoplasmic loop of the β4-subunit of rat nAChR.

The antibody is prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Biological Significance: ExpandCollapse

Nicotinic acetylcholine receptors (nAChRs) are ionotropic, cholinergic receptors that are divided into 2 types; muscle type and neuronal type. Neuronal nAChRs are pentameric ion channels consisting of 5 identical (homopentamers) or different (heteropentamers) subunits. Heteropentameric neuronal nAChRs mediate fast synaptic transmission in the autonomic nervous system. The predominant hetero-oligomeric nAChR in the CNS contain the subunits α4β2, whereas α3β4 prevail in the PNS. However, the expression of these subunits varies not only by region but also during development (Scholze et al 2011). In the brain, β2-containing receptors greatly outnumber receptors that contain β4 (McGehee & Role, 1995; Albuquerque et al., 2009), and in most brain regions, targeted deletion of the β2 subunit virtually abolishes [3H]-epibatidine binding and receptor autoradiography (Zoli et al., 1998) due to the absence of a β subunit required to form functional nAChRs (Champtiaux & Changeux, 2004).

Synonyms: ExpandCollapse

• acetylcholine receptor, nicotinic, beta 4 (neuronal) antibody
• ACHB4_HUMAN antibody
• AChR antibody
• Cholinergic receptor nicotinic beta 4 antibody
• Cholinergic receptor nicotinic beta polypeptide 4 antibody
• cholinergic receptor, nicotinic, beta polypeptide 4 antibody
• Chrnb4 antibody
• Neuronal acetylcholine receptor subunit beta-4 antibody
• Neuronal nicotinic receptor beta 4 subunit antibody


100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Scholze, P., Ciuraszkiewicz, A., Groessl, F., Orr‐Urtreger, A., McIntosh, J.M. and Huck, S., 2011. α4β2 nicotinic acetylcholine receptors in the early postnatal mouse superior cervical ganglion. Developmental Neurobiology, 71(5), pp.390-399. PMID: 10814726

McGehee, D.S. and Role, L.W., 1995. Physiological diversity of nicotinic acetylcholine receptors expressed by vertebrate neurons. Annual Review of Physiology, 57(1), pp.521-546. PMID: 10814726

Albuquerque, E.X., Pereira, E.F., Alkondon, M. and Rogers, S.W., 2009. Mammalian nicotinic acetylcholine receptors: from structure to function. Physiological Reviews, 89(1), pp.73-120. PMID: 10814726

Zoli, M., Léna, C., Picciotto, M.R. and Changeux, J.P., 1998. Identification of four classes of brain nicotinic receptors using β2 mutant mice. Journal of Neuroscience, 18(12), pp.4461-4472. PMID: 10814726

Champtiaux, N. and Changeux, J.P., 2004. Knockout and knockin mice to investigate the role of nicotinic receptors in the central nervous system. Progress in Brain Research, 145, pp.233-251. PMID: 10814726

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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