Rabbit Polyclonal AntibodyPooled Serum
- Affinity Purified from Pooled Serum
- Mouse, Rat
- WB 1:1000
- Rabbit Polyclonal
- Molecular Reference:
- ~52 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# 1470-nACB2, RRID:AB_2619712
The antigen is a fusion protein from the cytoplasmic loop of the β2-subunit of rat nAChR.
The antibody is prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.
Biological Significance: ExpandCollapse
Nicotinic acetylcholine receptors (nAChRs) are ionotropic, cholinergic receptors that are divided into 2 types; muscle type and neuronal type. Neuronal nAChRs are pentameric ion channels consisting of 5 identical (homopentamers) or different (heteropentamers) subunits. Heteropentameric neuronal nAChRs mediate fast synaptic transmission in the autonomic nervous system. The predominant hetero-oligomeric nAChR in the CNS contain the subunits α4β2, whereas α3β4 prevail in the PNS. However, the expression of these subunits varies not only by region but also during development (Scholze et al 2011). In the brain, β2-containing receptors greatly outnumber receptors that contain β4 (McGehee & Role, 1995; Albuquerque et al., 2009), and in most brain regions, targeted deletion of the β2 subunit virtually abolishes [3H]-epibatidine binding and receptor autoradiography (Zoli et al., 1998) due to the absence of a β subunit required to form functional nAChRs (Champtiaux & Changeux, 2004).