Affinity purified from pooled serum. Learn more.

NF-kB p65 (Ser316) Antibody

Catalog #: p1350-316 Category: Datasheet:

$109.00$365.00

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Rabbit Polyclonal Antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Bovine, Canine, Goat, Hamster, Human, Mouse, Pig, Rat
Applications:
WB 1:1,000
Species:
Rabbit Polyclonal
Gene Name:
RELA
Molecular Reference:
~65 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1350-316: RRID:AB_2756814
Antigen/Purification: ExpandCollapse

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser316 of mouse NF-kB p65.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Biological Significance: ExpandCollapse

p65, also known as RelA, is one of the five members of the NF-kB family. The p65 and p50 heterodimer is the predominant form of NF-kB, where p65 is the major subunit (Gilmore et al, 2006). There have been 13 identified phosphorylation sites on p65, including ser316 which is induced by IL-1β (Viatour et al, 2005 and Wang et al, 2015). Phosphorylation of ser316 is essential for NF-kB nuclear transportation, NF-kB-dependent gene regulation, cytokine/chemokine secretion, and other biological functions (Wang et al, 2015). Ser316 has also been demonstrated to co-phosphorylate with either Ser529 or Ser536 to regulate the majority of NF-kB targeted genes while having its own unique regulation function (Wang et al, 2015). CKI kinase specifically targets phosphorylation at ser316 on p65 (Wang et al, 2015). CKI is involved in regulation of membrane transport, cell division, DNA repair, and nuclear localization by phosphorylating its target proteins (Price et al, 2006).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

General References:

Gilmore, TD (2006) Introduction to NF-ϰB: players, pathways, perspecitives. Oncogene 25, 6680-6684.

Viatour P, Merville, MP, Bours V, Chariot A. (2005) Phosphorylation of NF- ϰB and IϰB proteins: implications in cancer and inflammation. Trends Biochem. Sci. 30, 43-52.

Wang B, Wei H, Prabhu L, Zhao W, Martin M, Hartley AV, and Lu T. (2015) Role of Novel Serine 316 Phosphorylation of the p65 Subunit of NF-ϰB in Differential Gene Regulation. J Biol Chem, 290(33):20336-47.

Price, MA. (2006) CKI, there’s more than one: casein kinase I family members in Wnt and Hedgehog signaling. Gene Dev. 20, 399-410.

 

Western blot of 20’ UV treated HeLa lysate showing specific immunolabeling of the ~65 kDa NF-kB p65 phosphorylated at Ser316 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is nearly eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).