Rabbit Polyclonal AntibodyPooled Serum
- Affinity Purified from Pooled Serum
- Bovine, Canine, Goat, Hamster, Human, Mouse, Pig, Rat
- WB 1:1,000
- Rabbit Polyclonal
- Gene Name:
- Molecular Reference:
- ~65 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# p1350-316: RRID:AB_2756814
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser316 of mouse NF-kB p65.
The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Biological Significance: ExpandCollapse
p65, also known as RelA, is one of the five members of the NF-kB family. The p65 and p50 heterodimer is the predominant form of NF-kB, where p65 is the major subunit (Gilmore et al, 2006). There have been 13 identified phosphorylation sites on p65, including ser316 which is induced by IL-1β (Viatour et al, 2005 and Wang et al, 2015). Phosphorylation of ser316 is essential for NF-kB nuclear transportation, NF-kB-dependent gene regulation, cytokine/chemokine secretion, and other biological functions (Wang et al, 2015). Ser316 has also been demonstrated to co-phosphorylate with either Ser529 or Ser536 to regulate the majority of NF-kB targeted genes while having its own unique regulation function (Wang et al, 2015). CKI kinase specifically targets phosphorylation at ser316 on p65 (Wang et al, 2015). CKI is involved in regulation of membrane transport, cell division, DNA repair, and nuclear localization by phosphorylating its target proteins (Price et al, 2006).