Neuron Specific Enolase (NSE) Antibody

Catalog #: 1520-NSE Categories: , , Datasheet:

$99.00$310.00

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Great neuronal marker for use in IHC!

 

Formulation:
Neat serum
Species Tested:
Bovine, Human, Mouse, Pig, Rat
Applications:
WB 1:2000IHC 1:500ICC 1:500
Species:
Rabbit polyclonal
Gene Name:
ENO2
Molecular Weight:
~47 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1520-NSE, RRID:AB_2492190
Antigen/Purification: ExpandCollapse

The antigen is a recombinant human NSE expressed in and purified from E. coli.

The antibody is unpurified neat serum.

Biological Significance: ExpandCollapse

Neuron specific enolase (NSE) is an enzyme which catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. It is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The three enolases have different cell type specific expression patterns, so that antibodies to them are useful cell type specific markers.(MacAlesse et al., 1988). NSE corresponds to ENO2 or enolase gamma and is heavily expressed in neuronal cells. ENO1 is also known as enolase alpha and as non-neuronal enolase. The third enolase, ENO3 or enolase beta, is expressed in muscle cells. Since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to this protein are therefore useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury (2).

Synonyms: ExpandCollapse

• 2 phospho D glycerate hydrolyase antibody
• 2-phospho-D-glycerate hydro-lyase antibody
• Eno 2 antibody
• ENO2 antibody
• ENOG antibody
• ENOG_HUMAN antibody
• Enolase 2 (gamma, neuronal) antibody
• Enolase 2 antibody
• Enolase 2 gamma neuronal antibody
• Enolase2 antibody
• Epididymis secretory protein Li 279 antibody
• Gamma enolase antibody
• Gamma-enolase antibody
• HEL S 279 antibody
• Neural enolase antibody
• Neuron specific enolase antibody
• Neuron specific gamma enolase antibody
• Neuron-specific enolase antibody
• neuronal enriched enolase antibody
• Neurone specific enolase antibody
• NSE antibody

Storage

100 µl liquid. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Immunocytochemistry

HEK 293 cells
E20 Rat mixed cortical neuron and glial cells

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

MacAlesse SM, Dunbar B, Fotherqill JE, Hinks LJ, Day IN (1988) Complete amino acid sequence of neurone-specific gamma isozyme of enolase (NSE) from human brain and comparison with the non-neuronal alpha form (NNE). Eur J Biochem. 178(2):413-17. PMID: 3208766

Begaz, T., Kyriacou, D. N., Segal, J. and Bazarian, J. J. Serum biochemical markers for post-concussion syndrome in patients with mild traumatic brain injury. J. Neurotrauma 23:1201-1210 (2006). PMID: 16928178

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Neuron Specific Enolase (NSE) Antibody
Western blot of rat cortical lysate showing specific immunolabeling of the ~47 kDa NSE protein.

Click here to view our Western blotting and lysate preparation protocols.

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