Neuron Specific Enolase (NSE) Antibody

Great neuronal marker for use in IHC! Our Neuron Specific Enolase rabbit polyclonal antibody is formulated as neat serum. Excellent for use in WB, IHC, and ICC.

Catalog #: 1520-NSE Categories: , , , Datasheet:


  • SizePrice
Neat serum
Species Tested:
Bovine, Chicken, Human, Mouse, Rat
WB 1:2000IHC 1:500ICC 1:500 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
Molecular Weight:
~47 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1520-NSE, RRID:AB_2492190
Antigen/Purification: ExpandCollapse

The antigen is a recombinant human NSE expressed in and purified from E. coli.

The antibody is unpurified neat serum.

Biological Significance: ExpandCollapse

Neuron specific enolase (NSE) is an enzyme which catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. It is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The three enolases have different cell type specific expression patterns, so that antibodies to them are useful cell type specific markers.(MacAlesse et al., 1988). NSE corresponds to ENO2 or enolase gamma and is heavily expressed in neuronal cells. ENO1 is also known as enolase alpha and as non-neuronal enolase. The third enolase, ENO3 or enolase beta, is expressed in muscle cells. Since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to this protein are therefore useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury (2).

Synonyms: ExpandCollapse

• 2 phospho D glycerate hydrolyase antibody
• 2-phospho-D-glycerate hydro-lyase antibody
• Eno 2 antibody
• ENO2 antibody
• ENOG antibody
• ENOG_HUMAN antibody
• Enolase 2 (gamma, neuronal) antibody
• Enolase 2 antibody
• Enolase 2 gamma neuronal antibody
• Enolase2 antibody
• Epididymis secretory protein Li 279 antibody
• Gamma enolase antibody
• Gamma-enolase antibody
• HEL S 279 antibody
• Neural enolase antibody
• Neuron specific enolase antibody
• Neuron specific gamma enolase antibody
• Neuron-specific enolase antibody
• neuronal enriched enolase antibody
• Neurone specific enolase antibody
• NSE antibody


100 µl liquid. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols


HEK 293 cells
E20 Rat mixed cortical neuron and glial cells

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

MacAlesse SM, Dunbar B, Fotherqill JE, Hinks LJ, Day IN (1988) Complete amino acid sequence of neurone-specific gamma isozyme of enolase (NSE) from human brain and comparison with the non-neuronal alpha form (NNE). European Journal of Biochemistry. 178(2):413-17. PMID: 3208766

Begaz, T., Kyriacou, D. N., Segal, J. and Bazarian, J. J. Serum biochemical markers for post-concussion syndrome in patients with mild traumatic brain injury. Journal of Neurotrauma 23:1201-1210 (2006). PMID: 16928178

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
Leave a Reply

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

Learn How Your Review May Be Used

You may also like…