Neurofilament M Antibody, Chicken

Excellent marker of neuronal accumulations in IHC! Our Neurofilament M chicken polyclonal antibody is total IgY fraction. Excellent for use in WB, IHC, and ICC.

Catalog #: 1455-NFM Categories: , , , Datasheet:
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$115.00 – $335.00

  • SizePrice
Clear
Formulation:
Total IgY fraction
Species Tested:
Bovine, Chicken, Human, Mouse, Pig, Rat
Applications:
WB 1:5000IHC 1:1000ICC 1:1000 Don't see your application?
Host Species:
Chicken Polyclonal
Gene Name:
NEFM
Molecular Weight:
~145 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1455-NFM, RRID:AB_2492164
Antigen/Purification: ExpandCollapse

The antigen is a recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli.

The antibody is  total IgY fraction.

Biological Significance: ExpandCollapse

Neurofilaments are the 10 nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (Harris et al., 1991). NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig’s disease) and Alzheimer’s disease (Hu et al., 2002).

Synonyms: ExpandCollapse

• 150 kDa Neurofilament Medium antibody
• 160 kDa neurofilament protein antibody
• Medium polypeptide 150kDa antibody
• NEF3 antibody
• Neurofilament 3 antibody
• Neurofilament 3, medium antibody
• Neurofilament medium polypeptide antibody
• Neurofilament protein M antibody
• Neurofilament triplet M protein antibody
• Neurofilament-3 (150 kD medium) antibody
• NF-M antibody
• NFM antibody

Storage

100 µl liquid containing 10mM sodium azide. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Immunocytochemistry

Cultured rat neurons

Immunohistochemistry

Rat Cerebellum

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. Journal of Neuroscience Research 30:47-62. PMID: 1724473

Hu YY, He SS, Wang XC, Duan QH, Khatoon S, Igbal K, Grundke-Igbal I, Wang JZ (2002) Elevated levels of phosphorylated neurofilament proteins in cerebrospinal fluid of Alzheimer disease patients. Neuroscience Letters 320(3):156-60. PMID: 11852185

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Neurofilament M Antibody, Chicken
Western blot of rat cortical lysate showing specific immunolabeling of the ~145 kDa NF-M protein.

Click here to view our Western blotting and lysate preparation protocols.
Neurofilament M Antibody, Chicken
Immunostaining of cultured rat neurons and glia stained with anti-NFM antibody (catalog #1455-NFM, red, 1:1000) and DNA is stained blue.

Download the protocol used for this image:
Immunofluorescence of a section of rat cerebellum labeled with anti-Neurofilament-M (cat #1455-NFM, 1:1000, red) and anti-CNP(green). The anti-NFM labels the network of axons of basket neurons and other neurons. The blue is DAPI staining of nuclear DNA.

Download the protocol used for this image:

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