Neurofilament L Antibody, Chicken

Great for use in IHC on neurofilament accumulations! Our Neurofilament L chicken polyclonal antibody is total IgY fraction. Excellent for use in WB, IHC, and ICC.

Catalog #: 1453-NFL Categories: , , , Datasheet:
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$115.00 – $335.00

  • SizePrice
Clear
Formulation:
Total IgY fraction
Species Tested:
Bovine, Chicken, Human, Mouse, Pig, Rat
Applications:
WB 1:10000IHC 1:2000ICC 1:2000-1:5000 Don't see your application?
Host Species:
Chicken Polyclonal
Gene Name:
NEFL
Molecular Weight:
~68 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1453-NFL, RRID:AB_2492162
Antigen/Purification: ExpandCollapse

The antigen is a preparation of bovine spinal cord intermediate filaments.

The antibody is total IgY fraction.

Biological Significance: ExpandCollapse

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease (2).

Synonyms: ExpandCollapse

• 68 kDa neurofilament protein antibody
• 68kDa Neurofilament antibody
• 68kDa neurofilament protein antibody
• CMT1F antibody
• CMT2E antibody
• FLJ53642 antibody
• Light molecular weight neurofilament protein antibody
• NEFL antibody
• Neurofilament light antibody
• Neurofilament light polypeptide 68kDa antibody
• Neurofilament light polypeptide antibody
• Neurofilament protein, light chain antibody
• Neurofilament subunit NF L antibody
• Neurofilament triplet L protein antibody
• NF-L antibody
• NF68 antibody
• NFL antibody
• NFL_HUMAN antibody

Storage

100 µl liquid containing 10mM sodium azide. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Immunocytochemistry

Mixed neuron and glia cultured cells

Immunohistochemistry

Rat Cerebellum

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. Journal of Neuroscience Research 30:47-62. PMID: 10841809

Mersiyanova IV, Perepelov AV, Polyakov AV, Sitnikov VF, Dadali EL, Oparin RB, Petrin AN and Evgrafov OV. (2000) A new variant of Charcot-Marie-Tooth disease type 2 is probably the result of a mutation in the neurofilament-light gene. American Journal of Human Genetics 67:37-46. PMID: 1724473

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Neurofilament L Antibody, Chicken
Western blot of rat cortical lysate showing specific immunolabeling of the ~68 kDa NF-L protein.

Click here to view our Western blotting and lysate preparation protocols.
Neurofilament L Antibody, Chicken
Immunostaining of mixed cultured rat neurons and glia stained with anti-NFL antibody (catalog #1453-NFL, red, 1:5000). The NF-L protein is assembled into neurofilaments which are found throughout the axons, dendrites, and perikarya as shown above.

Download the protocol used for this image:
Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament-L (cat #1453-NFL, 1:2000, green) and labeling of FOX3/NeuN(cat #583-FOX3, 1:5000, red). The anti-NFL strongly labels the axons of basket calls and perikarya and processes of neuronal cells. The anti-FOX3/NeuN labels the nuclei and proximal cytoplasm of neurons. The blue is DAPI staining of nuclear DNA.

Download the protocol used for this image:

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