Neurofilament H Antibody, Mouse

Great for studying neurofilament accumulations in IHC! Our Neurofilament H mouse monoclonal antibody is Protein G purified. Excellent for use in WB, IHC, and ICC.

Catalog #: 1450-NFH Categories: , , , Datasheet:

$125.00$390.00

  • SizePrice
Clear
Formulation:
Protein G purified tissue culture supernatant
Species Tested:
Bovine, Chicken, Human, Mouse, Pig, Rat
Expected Reactivity:
Most mammals
Applications:
WB 1:5000IHC 1:500-1:1000ICC 1:500-1:1000 Don't see your application?
Host Species:
Mouse Monoclonal
Gene Name:
NEFH
Molecular Weight:
~200 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1450-NFH, RRID:AB_2492159
Antigen/Purification: ExpandCollapse

The antigen is a purified bovine neurofilament H.

The antibody is Protein G purified tissue culture supernatant.

Biological Significance: ExpandCollapse

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig’s disease) (2) and Alzheimer’s disease (3).

Synonyms: ExpandCollapse

• 200 kDa neurofilament protein antibody
• CMT2CC antibody
• Nefh antibody
• Neurofilament heavy polypeptide 200kDa antibody
• Neurofilament heavy polypeptide antibody
• Neurofilament triplet H protein antibody
• NF H antibody
• NF-H antibody
• NFH antibody
• NFH_HUMAN antibody

Storage

100 µl in PBS + 10 mM NaN3. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62. PMID: 1724473

Mendonca DM, Chimelli L, Martinez AM. (2005) Quantitative evidence for neurofilament heavy subunit aggregation in motor neurons of spinal cords of patients with amyotrophic lateral sclerosis. Braz J Med Biol Res. 38(6):925-933. PMID: 15933787

Hu YY, He SS, Wang XC, Duan QH, Khatoon S, Igbal K, Grundke-Igbal I, Wang JZ (2002) Elevated levels of phosphorylated neurofilament proteins in cerebrospinal fluid of Alzheimer disease patients. Neurosci Lett 320(3):156-60. PMID: 11852185

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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