Anti-Neurofilament NF-H Antibody

Our Anti-Neurofilament NF-H primary antibody from PhosphoSolutions is chicken polyclonal. It detects all known mammal and chicken Neurofilament NF-H and is total IgY fraction. It is great for use in WB, IHC, ICC.

Western blot of rat cortical lysate showing specific immunolabeling of the ~200 kDa NF-H protein.
Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament H (cat. 1451-NFH, 1:25,000, red) in nuclei of neurons and specific labeling of GFAP (cat. 620-GFAP, 1:5000, green) in astrocytes and other glial cells, and DAPI staining of nuclear DNA.
Immunofluorescence of a section of rat cerebellum showing specific labeling of the axons with anti-neurofilament H antibody (cat. 1451-NFH, green, 1:25,000). The section is colabeled with anti-calbindin (cat. 302-CALB , red, 1:1000) labeling the dendrites of Purkinje cells. The blue is DAPI staining nuclear DNA.
Immunostaining of mixed cultured rat neurons and glia stained with anti-NFH antibody (cat. 1451-NFH, red, 1:25,000) and anti-NFL antibody (cat. 1452-NFL, green, 1:100).
Immunofluorescence of a section of adult rat frontal cortex labeled with anti-Neurofilament-M (cat. 1454-NFM, 1:5000, red) and anti-Neurofilament-H (cat. 1451-NFH, 1:5000, green). The anti-NFM specifically labels neuron cell bodies and dendrites of pyramidal neurons, while the anti-NFH labels the network of neuronal axons only. The blue is Hoechst staining of nuclear DNA.
Immunofluorescence of a section of rat spinal cord labeled with anti-UCHL1 (cat. 2060-UCHL1, 1:500, red) and anti-Neurofilament H (cat. 1451-NFH, 1:25,000, green). The large cells are alpha-motorneurons and UCHL1 fills the cytoplasm of their perikarya and dendrites.
Western blot of rat cortical lysate showing specific immunolabeling of the ~200 kDa NF-H protein.
Western blot of rat cortical lysate showing specific immunolabeling of the ~200 kDa NF-H protein.
Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament H (cat. 1451-NFH, 1:25,000, red) in nuclei of neurons and specific labeling of GFAP (cat. 620-GFAP, 1:5000, green) in astrocytes and other glial cells, and DAPI staining of nuclear DNA.
Immunofluorescence of a section of rat cerebellum showing specific labeling of the axons with anti-neurofilament H antibody (cat. 1451-NFH, green, 1:25,000). The section is colabeled with anti-calbindin (cat. 302-CALB , red, 1:1000) labeling the dendrites of Purkinje cells. The blue is DAPI staining nuclear DNA.
Immunostaining of mixed cultured rat neurons and glia stained with anti-NFH antibody (cat. 1451-NFH, red, 1:25,000) and anti-NFL antibody (cat. 1452-NFL, green, 1:100).
Immunofluorescence of a section of adult rat frontal cortex labeled with anti-Neurofilament-M (cat. 1454-NFM, 1:5000, red) and anti-Neurofilament-H (cat. 1451-NFH, 1:5000, green). The anti-NFM specifically labels neuron cell bodies and dendrites of pyramidal neurons, while the anti-NFH labels the network of neuronal axons only. The blue is Hoechst staining of nuclear DNA.
Immunofluorescence of a section of rat spinal cord labeled with anti-UCHL1 (cat. 2060-UCHL1, 1:500, red) and anti-Neurofilament H (cat. 1451-NFH, 1:25,000, green). The large cells are alpha-motorneurons and UCHL1 fills the cytoplasm of their perikarya and dendrites.
Chicken
ICC, IHC, WB
Chicken

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from PhosphoSolutions Datasheet
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SKU: 1451-NFH

Volume: 100 µL
Price:
$375

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