Neurofilament H Antibody, Chicken

Our Neurofilament H chicken polyclonal antibody is total IgY fraction. Excellent for use in WB, IHC, and ICC. Great for staining neurofilament accumulations seen in AD and ALS.

Catalog #: 1451-NFH Categories: , , ,

$115.00$335.00

  • SizePrice
Clear
Formulation:
Total IgY fraction
Species Tested:
Bovine, Canine, Human, Mouse, Pig, Rat
Expected Reactivity:
Most mammals
Applications:
WB 1:50000IHC 1:5000-1:25,000ICC 1:25,000 Don't see your application?
Host Species:
Chicken Polyclonal
Gene Name:
NEFH
Molecular Weight:
~200 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1451-NFH, RRID:AB_2492160
Antigen/Purification: ExpandCollapse

The antigen is a purified bovine NF-H.

The antibody is total IgY fraction.

Biological Significance: ExpandCollapse

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (Harris et al., 1991). NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig’s disease) (Mendonca et al., 2005) and Alzheimer’s disease (Hu et al., 2002).

Synonyms: ExpandCollapse

• 200 kDa neurofilament protein antibody
• CMT2CC antibody
• Nefh antibody
• Neurofilament heavy polypeptide 200kDa antibody
• Neurofilament heavy polypeptide antibody
• Neurofilament triplet H protein antibody
• NF H antibody
• NF-H antibody
• NFH antibody
• NFH_HUMAN antibody

Storage

100 µl liquid containing 10mM sodium azide. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Immunocytochemistry

Rat neuron and glia cells

Immunohistochemistry

Rat Cerebellum

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62.PMID: 1724473

Mendonca DM, Chimelli L, Martinez AM. (2005) Quantitative evidence for neurofilament heavy subunit aggregation in motor neurons of spinal cords of patients with amyotrophic lateral sclerosis. Braz J Med Biol Res. 38(6):925-933. PMID: 15933787

Hu YY, He SS, Wang XC, Duan QH, Khatoon S, Igbal K, Grundke-Igbal I, Wang JZ (2002) Elevated levels of phosphorylated neurofilament proteins in cerebrospinal fluid of Alzheimer disease patients. Neurosci Lett 320(3):156-60. PMID: 11852185

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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