Nestin Antibody

Catalog #: 1435-NES Categories: , , , Datasheet:

$109.00$365.00

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Excellent marker of astrocytes and neuronal stem cells!

 

Formulation:
Protein G Purified
Species Tested:
Human, Mouse, Rat
Applications:
WB 1:2000IHC 1:500ICC 1:500
Species:
Mouse monoclonal
Gene Name:
NES
Molecular Weight:
~220-240 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1435-NES, RRID:AB_2492158
Antigen/Purification: ExpandCollapse

The antigen is a recombinant partial segment of human nestin expressed in and purified from E.coli.

The antibody is Protein G purified.

Biological Significance: ExpandCollapse

Nestin is a member of the class IV intermediate filament protein family which is expressed in neuronal stem cells (Lendahl et al., 1990). Nestin was originally identified as a result of the production of a series of monoclonal antibodies directed against epitopes expressed on formalin fixed embryo day 15 rat spinal cord (Hockfield et al., 1985)). One of these antibodies, called Rat 401, stained fibrous profiles in the developing nervous system, but not in the mature nervous system. By screening bacteriophage expression libraries with Rat 401, Lendahl et al. were able to isolate a cDNA encoding the protein to which Rat 401 antibody bound. The protein proved to be an unusual member of the intermediate filament family, containing an alpha-helical region homologous to that found in keratin and neurofilament subunits.

Synonyms: ExpandCollapse

• ESTM 46 antibody
• FLJ 21841 antibody
• FLJ21841 antibody
• Intermediate filament protein antibody
• Nbla00170 antibody
• nes antibody
• NEST_HUMAN antibody
• Nestin antibody

Storage

100 µl in PBS + 50% glycerol + 5 mM NaN3. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunocytochemistry

Neonatal rat cultured neurons and glia
E20 Rat cortical neuron-glial cell culture

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunohistochemistry: Rat


Immunohistochemistry: Rat
PMID Dilution Publication
28641285 Not listed Thakur, V., et al. 2017. Role of Glycyrrhizin in the Reduction of Inflammation in Diabetic Kidney Disease. Nephron. 137(2):137-147.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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