Affinity purified from pooled serum. Learn more.

NCC (Thr53) Antibody

Catalog #: p1311-53 Categories: , Datasheet:

$109.00$365.00

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Rabbit Polyclonal Antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Reactivity:
Mouse, Guinea Pig, Hamster
Applications:
WB 1:1000IF 1:100 (McCormick et al. 2011)
Species:
Rabbit Polyclonal
Molecular Reference:
~160 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1311-53, RRID:AB_2650477
Antigen/Purification: ExpandCollapse

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr53 of mouse NCC.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Biological Significance: ExpandCollapse

The thiazide-sensitive sodium chloride cotransporter, NCC, is the major NaCl transport protein in the distal convoluted tubule (DCT) and plays an important role in maintaining blood pressure (Rosenbaek et al., 2014, Feng et al., 2015). Phosphorylation of NCC at Thr-53, Thr-58, and Ser-71 is an essential mediator of NCC function (Rosenbaek et al., 2014). NCC is constitutively cycled to the plasma membrane, and upon stimulation, it can be phosphorylated to both increase NCC activity and decrease NCC endocytosis, together increasing NaCl transport in the DCT (Feng et al., 2015).

Synonyms: ExpandCollapse

• FLJ96318 antibody
• Na Cl cotransporter antibody
• Na Cl symporter antibody
• Na-Cl symporter antibody
• NaCl electroneutral thiazide sensitive cotransporter antibody
• NCCT antibody
• SLC12A3 antibody
• S12A3_HUMAN antibody
• slc12a3 antibody
• Solute carrier family 12 (sodium/chloride transporters) member 3 antibody
• Solute carrier family 12 member 3 antibody
• Thiazide sensitive Na Cl cotransporter antibody
• Thiazide sensitive sodium chloride cotransporter antibody
• Thiazide-sensitive sodium-chloride cotransporter antibody
• TSC antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific References

Khamaysi, A., Anbtawee-Jomaa, S., Fremder, M., Eini-Rider, H., Shimshilashvili, L., Aharon, S., Aizenshtein, E., Shlomi, T., Noguchi, A., Springer, D. and Moe, O.W., 2019. Systemic Succinate Homeostasis and Local Succinate Signaling Affect Blood Pressure and Modify Risks for Calcium Oxalate Lithogenesis. Journal of the American Society of Nephrology, pp.ASN-2018030277. PMID: 30728179

Duan, X.P., Gu, L., Xiao, Y., Gao, Z.X., Wu, P., Zhang, Y.H., Meng, X.X., Wang, J.L., Zhang, D.D., Lin, D.H. and Wang, W.H., 2019. Norepinephrine-Induced Stimulation of Kir4. 1/Kir5. 1 Is Required for the Activation of NaCl Transporter in Distal Convoluted Tubule. Hypertension,73:112-120. PMID: 30571558

Saritas, T., Puelles, V.G., Su, X.T., McCormick, J.A., Welling, P.A. and Ellison, D.H., 2018. Optical Clearing in the Kidney Reveals Potassium-Mediated Tubule Remodeling. Cell reports, 25(10), pp.2668-2675. PMID: 30517856

Xu, J., Barone, S., Zahedi, K., Brooks, M. and Soleimani, M., 2018. Slc4a8 in the Kidney: Expression, Subcellular Localization and Role in Salt Reabsorption. Cellular Physiology and Biochemistry, 50(4), pp.1361-1375. PMID: 30355950

Cherezova, A., Tomilin, V., Buncha, V., Zaika, O., Ortiz, P.A., Mei, F., Cheng, X., Mamenko, M. and Pochynyuk, O., 2018. Urinary concentrating defect in mice lacking Epac1 or Epac2. The FASEB Journal, pp.fj-201800435R. PMID: 30252533

Tutakhel, O.A., Bianchi, F., Smits, D.A., Bindels, R.J., Hoenderop, J.G. and van der Wijst, J., 2018. Dominant functional role of the novel phosphorylation site S811 in the human renal NaCl cotransporter. The FASEB Journal, pp.fj-201701047R. PMID: 29547703

Palygin, O., Levchenko, V., Ilatovskaya, D.V., Pavlov, T.S., Pochynyuk, M., Jacob, H.J., Geurts, A.M., Hodges, M.R. and Staruschenko, A., 2017. Essential role of K ir 5.1 channels in renal salt handling and blood pressure control. JCI Insight, 2(18). PMID: 28931751

Terker AS, Yarbrough B, Ferdaus MZ, Lazelle RA, Erspamer KJ, Meermeier NP, Park HJ, McCormick JA, Yang CL, Ellison D3. (2016) Direct and Indirect Mineralocorticoid Effects Determine Distal Salt Transport. J Am Soc Nephrol. (8):2436-45.  PMID: 26712527 

Terker AS, Zhang C, McCormick JA, Lazelle RA, Zhang C, Meermeier NP, Siler D, Park HJ, Fu Y, Cohen DM, Weinstein AM, Wang WH, Yang CL, Ellison DH. (2015) Potassium modulates electrolyte balance and blood pressure through effects on distal cell voltage and chloride. Cell Metab. (1):39-50. PMID: 25565204

McCormick, J. A., Nelson, J. H., Yang, C. L., Curry, J. N., & Ellison, D. H. (2011). Overexpression of the sodium chloride cotransporter is not sufficient to cause familial hyperkalemic hypertension. Hypertension, 58(5), 888-894. PMID: 21896937

 

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NCC thr53 antibody
Western blot of mouse kidney lysate showing specific immunolabeling of the ~160 kDa NCC protein phosphorylated at Thr53 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
p1311-53 NCC thr53 IF image
Immunostaining of PFA perfused frozen kidney sections from WT and NCC KO mice showing specific labeling of the NCC protein phosphorylated at Thr53 on the left and the absence of staining in the KO on the right. (Photo courtesy of Lauren Miller, Ellison Lab, OHSU.)

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