Affinity purified from pooled serum. Learn more.

NCC (Thr53) Antibody

Catalog #: p1311-53 Categories: , Datasheet:

$109.00$365.00

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Rabbit Polyclonal Antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Mouse, Guinea Pig, Hamster
Applications:
WB 1:1,000IF 1:100 (McCormick et al., 2011)
Species:
Rabbit Polyclonal
Molecular Reference:
~160 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1311-53, RRID:AB_2650477
Antigen/Purification: ExpandCollapse

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr53 of mouse NCC.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Biological Significance: ExpandCollapse

The thiazide-sensitive sodium chloride cotransporter, NCC, is the major NaCl transport protein in the distal convoluted tubule (DCT) and plays an important role in maintaining blood pressure (Rosenbaek et al., 2014, Feng et al., 2015). Phosphorylation of NCC at Thr-53, Thr-58, and Ser-71 is an essential mediator of NCC function (Rosenbaek et al., 2014). NCC is constitutively cycled to the plasma membrane, and upon stimulation, it can be phosphorylated to both increase NCC activity and decrease NCC endocytosis, together increasing NaCl transport in the DCT (Feng et al., 2015).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Duan, X.P., Gu, L., Xiao, Y., Gao, Z.X., Wu, P., Zhang, Y.H., Meng, X.X., Wang, J.L., Zhang, D.D., Lin, D.H. and Wang, W.H., 2019. Norepinephrine-Induced Stimulation of Kir4. 1/Kir5. 1 Is Required for the Activation of NaCl Transporter in Distal Convoluted Tubule. Hypertension,73:112-120. PMID: 30571558

Xu, J., Barone, S., Zahedi, K., Brooks, M. and Soleimani, M., 2018. Slc4a8 in the Kidney: Expression, Subcellular Localization and Role in Salt Reabsorption. Cellular Physiology and Biochemistry, 50(4), pp.1361-1375. PMID: 30355950

Cherezova, A., Tomilin, V., Buncha, V., Zaika, O., Ortiz, P.A., Mei, F., Cheng, X., Mamenko, M. and Pochynyuk, O., 2018. Urinary concentrating defect in mice lacking Epac1 or Epac2. The FASEB Journal, pp.fj-201800435R. PMID: 30252533

Tutakhel, O.A., Bianchi, F., Smits, D.A., Bindels, R.J., Hoenderop, J.G. and van der Wijst, J., 2018. Dominant functional role of the novel phosphorylation site S811 in the human renal NaCl cotransporter. The FASEB Journal, pp.fj-201701047R. PMID: 29547703

Palygin, O., Levchenko, V., Ilatovskaya, D.V., Pavlov, T.S., Pochynyuk, M., Jacob, H.J., Geurts, A.M., Hodges, M.R. and Staruschenko, A., 2017. Essential role of K ir 5.1 channels in renal salt handling and blood pressure control. JCI Insight, 2(18). PMID: 28931751

Terker AS, Yarbrough B, Ferdaus MZ, Lazelle RA, Erspamer KJ, Meermeier NP, Park HJ, McCormick JA, Yang CL, Ellison D3. (2016) Direct and Indirect Mineralocorticoid Effects Determine Distal Salt Transport. J Am Soc Nephrol. (8):2436-45.  PMID: 26712527 

Terker AS, Zhang C, McCormick JA, Lazelle RA, Zhang C, Meermeier NP, Siler D, Park HJ, Fu Y, Cohen DM, Weinstein AM, Wang WH, Yang CL, Ellison DH. (2015) Potassium modulates electrolyte balance and blood pressure through effects on distal cell voltage and chloride. Cell Metab. (1):39-50. PMID: 25565204

McCormick, J. A., Nelson, J. H., Yang, C. L., Curry, J. N., & Ellison, D. H. (2011). Overexpression of the sodium chloride cotransporter is not sufficient to cause familial hyperkalemic hypertension. Hypertension, 58(5), 888-894. PMID: 21896937

 

NCC thr53 antibody
Western blot of mouse kidney lysate showing specific immunolabeling of the ~160 kDa NCC protein phosphorylated at Thr53 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
p1311-53 NCC thr53 IF image
Immunostaining of PFA perfused frozen kidney sections from WT and NCC KO mice showing specific labeling of the NCC protein phosphorylated at Thr53 on the left and the absence of staining in the KO on the right. (Photo courtesy of Lauren Miller, Ellison Lab, OHSU.)

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