Rabbit Polyclonal AntibodyPooled Serum
- Affinity Purified from Pooled Serum
- Mouse, Guinea Pig, Hamster
- WB 1:1,000IF 1:100 (McCormick et al., 2011)
- Rabbit Polyclonal
- Molecular Reference:
- ~160 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# p1311-53, RRID:AB_2650477
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr53 of mouse NCC.
The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Biological Significance: ExpandCollapse
The thiazide-sensitive sodium chloride cotransporter, NCC, is the major NaCl transport protein in the distal convoluted tubule (DCT) and plays an important role in maintaining blood pressure (Rosenbaek et al., 2014, Feng et al., 2015). Phosphorylation of NCC at Thr-53, Thr-58, and Ser-71 is an essential mediator of NCC function (Rosenbaek et al., 2014). NCC is constitutively cycled to the plasma membrane, and upon stimulation, it can be phosphorylated to both increase NCC activity and decrease NCC endocytosis, together increasing NaCl transport in the DCT (Feng et al., 2015).