Myelin Basic Protein (MBP) Antibody

Catalog #: 1120-MBP Categories: , , Datasheet:

$99.00$310.00

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Works great as a marker in IHC!

Formulation:
Total IgY fraction
100% Homology:
Rat, Mouse, Human, Bovine
Applications:
WB 1:5,000 IF 1:500
Species:
Chicken polyclonal
Gene Name:
MBP
Molecular Reference:
~18 kDa and ~22 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1120-MBP, RRID:AB_2492157
Antigen/Purification: ExpandCollapse

The antigen is three peptide sequences conserved in higher vertebrate MBP proteins.

The antibody is total IgY fraction.

Biological Significance: ExpandCollapse

Myelin Basic Protein (MBP) is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago (Eylar et al., 1971). The protein is made by oligodendrocytes in the central nervous system, so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells (Marty et al., 2002). In the central nervous system there are there different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 18.5, and 17.2 kDa. Since the two lower molecular weight forms are very close in molecular size, MBP antibodies typically show two bands on Western blots, one at about 22 kDa and another at about 18 kDa.

Storage

Liquid. Contains 10 mM sodium azide.

For long term storage –20° C is recommended in undiluted aliquots. Stable at –20° C for at least one year after date of receipt. Avoid freeze/thaw cycles.

Product Specific Reference

Gatto, R.G., Li, W., Gao, J. and Magin, R.L., 2018. In vivo diffusion MRI detects early spinal cord axonal pathology in a mouse model of amyotrophic lateral sclerosis. NMR in Biomedicine, Aug;31(8):e3954. PMID: 30117615

Gatto, R.G., Li, W. and Magin, R.L., 2018. Diffusion Tensor Imaging Identifies Presymptomatic Axonal Degeneration in the Spinal Cord of ALS Mice. Brain Research 1679, 45–52. PMID: 29175489

 

Myelin Basic Protein (MBP) Antibody
Western blot of rat cortical lysate showing specific immunolabeling of the ~18 kDa and ~22 kDa MBP protein.
Myelin Basic Protein (MBP) Antibody
Rat mixed neuron/glial cultures stained with chicken Myelin Basic Protein (red). Blue is a DNA stain. Note that the Myelin Basic Protein antibody stains an oligodendrocyte and some membrane shed from this cell. Other cells in the field include neurons, astrocytes, microglia and fibroblasts, all of which are completely negative for myelin basic protein.

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