Affinity purified from pooled serum. Learn more.

Munc18-1 (Ser241) Antibody

Catalog #: p1305-241 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Rat, Mouse, Human, Bovine, Non-human primate, Sheep, Canine
Applications:
WB 1:1,000
Species:
Rabbit
Molecular Reference:
~65 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1305-241, RRID:AB_2492155
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser241 of mouse Munc18-1.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho- peptide affinity columns.

Biological Significance: ExpandCollapse

Munc-18 (mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Munc18-1 is thought to be regulated by phosphorylation.

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Wang, W., Jia, Y., Pham, D.T., Palmer, L.C., Jung, K.M., Cox, C.D., Rumbaugh, G., Piomelli, D., Gall, C.M. and Lynch, G., 2017. Atypical Endocannabinoid Signaling Initiates a New Form of Memory-Related Plasticity at a Cortical Input to Hippocampus. Cerebral Cortex, pp.1-14. PMID: 28520937

Meijer, M., Cijsouw, T., Toonen, R. F., & Verhage, M. (2015). Synaptic Effects of Munc18-1 Alternative Splicing in Excitatory Hippocampal Neurons. PloS one, 10(9), e0138950. PMID: 26407320

Munc18-1 Ser241 Antibody
Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~65 kDa Munc18-1 protein phosphorylated at Ser241 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

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