Affinity purified from pooled serum. Learn more.

Munc-18 (Ser513) Antibody

We are the original manufacturer of our Munc-18 (Ser513) rabbit polyclonal phosphospecific antibody, affinity purified from pooled serum. Optimized in WB and IHC.

Catalog #: p1305-515 Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Rat
Expected Reactivity:
Mouse, Human, Xenopus, Bovine, Chicken, Non-human primate, Canine
Applications:
WB 1:1000ICC 1:400 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
STXBP1
Molecular Weight:
~65 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1305-515, RRID:AB_2492156
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser513 of Munc-18.

The antibody is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Munc-18 (mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Unc-18 is thought to be regulated by PKC phosphorylation of Ser515 in C. Elegans (Sassa et al., 1996). Ser513 is the mammalian homologue of Ser515 in C. Elegans.

Synonyms: ExpandCollapse

• Alternative names
• FLJ37475 antibody
• Munc 18 1 antibody
• Munc 18a antibody
• MUNC18 1 antibody
• N-Sec1 antibody
• Neuronal SEC1 antibody
• NSec1 antibody
• p67 antibody
• Protein unc-18 homolog 1 antibody
• Protein unc-18 homolog A antibody
• Rb sec1 antibody
• RBSEC1 antibody
• STXB1_HUMAN antibody
• STXBP1 antibody
• Syntaxin binding protein 1 antibody
• Syntaxin-binding protein 1 antibody
• Unc 18 homolog antibody
• Unc 18A antibody
• Unc-18A antibody
• Unc18 1 antibody
• UNC18 antibody
• Unc18-1 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Rat


Western Blot: Rat
PMID Dilution Publication
18519737 not listed Leal-Ortiz, S., et al. 2008 Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis. Journal of Cell Biology, 181: 831- 846.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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