Rabbit polyclonal antibodyPooled Serum
- 100 µl
- Affinity Purified from Pooled Serum
- Rat, Mouse, Human, Xenopus, Bovine, Chicken, Non-human primate, Canine
- WB 1:1,000IHC 1:400
- Molecular Reference:
- ~65 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# p1305-515, RRID:AB_2492156
The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser513 of Munc-18.
The antibody is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Biological Significance: ExpandCollapse
Munc-18 (mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Unc-18 is thought to be regulated by PKC phosphorylation of Ser515 in C. Elegans (Sassa et al., 1996). Ser513 is the mammalian homologue of Ser515 in C. Elegans.