Affinity purified from pooled serum. Learn more.

Mnk2b Antibody

Catalog #: 1181-Mnk2b Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Human, Non-human primate
Applications:
WB 1:1000
Species:
Rabbit Polyclonal
Molecular Reference:
~47 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1181-Mnk2b, RRID:AB_2631043
Antigen/Purification: ExpandCollapse

Peptide from the C-terminal region, specific to the human Mnk2b isoform.  The sequence of the antigen has no homology with Mnk1 or Mnk2a.

Prepared from pooled rabbit serum by affinity purification via chromatography on peptide immunogen column.

 

Biological Significance: ExpandCollapse

MAP kinase-interacting kinases Mnk1 and Mnk2, are activated by the MAP kinases, ERK and p38 (Waskiewicz et al., 1997).  Additionally, Mnk1 and Mnk2 have been shown to phosphorylate the translation initiation factor eIF4E (Fukunaga and Hunter 1997; Waskiewicz et al., 1997).   Alternative splicing of the MKNK2 gene results in two isoforms with different C-termini: one that has a MAPK-binding domain, Mnk2a, and one that does not, Mnk2b (Parra et al., 2005).  Recent studies have indicated that Mnk2a has tumor suppressive activity while Mnk2b is pro-oncogenic (Maimon et al., 2014).

Storage

Shipped on blue ice. Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

General References:

Fukunaga, R., & Hunter, T. (1997) A New MAP Kinase-Activated Protein Kinase Isolated by a Novel Expression Screening Method to Identify Protein Kinase Substrates. EMBO J. Apr 15;16(8):1921-33.

Maimon  A, Mogilevsky M, Shilo A, Golan-Gerstl R, Obiedat A, Ben-Hur V, Lebenthal-Loinger I, Stein I, Reich R, Beenstock J, Zehorai E, Andersen CL, Thorsen K, Orntoft TF, Davis RJ, Davidson B, Mu D, Karni R (2014) Mnk2 Alternative Splicing Modulates the p38-MAPK Pathway and Impacts Ras-Induced Transformation. Cell Reports 7, 501-513.

Parra JL, Buxadé M, Proud CG (2005) Features of the Catalytic Domains and C Termini of the MAPK Signal-integrating Kinases Mnk1 and Mnk2 Determine Their Differing Activities and Regulatory Properties. J. Biol. Chem. 280, 37623-37633.

Waskiewicz AJ, Flynn A, Proud CG, Cooper JA (1997) Mitogen-activated Protein Kinases Activate the Serine/Threonine Kinases Mnk1 and Mnk2. EMBO J. 16,1909-1920.

 

Mnk2b antibody
Western blot of HeLa lysate showing specific immunolabeling of the ~47 kDa Mnk2b isoform in the first lane (-). Specificity is shown in the third lane (Mnk2b) where immunolabeling is blocked by preadsorption of the Mnk2b peptide used as the antigen, but not by the Mnk2a peptide in the second lane.

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