Affinity purified from pooled serum. Learn more.

Mnk2a Antibody

We are the original manufacturer of our Mnk2a rabbit polyclonal antibody, affinity purified from pooled serum. Optimized in WB.

Catalog #: 1180-Mnk2a Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human
Expected Reactivity:
Non-human primate
Applications:
WB 1:500 Don't see your application?
Host Species:
Rabbit Polyclonal
Molecular Weight:
~52 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1180-Mnk2a, RRID:AB_2631038
Antigen/Purification: ExpandCollapse

The antigen is a peptide from the C-terminal region, specific to the human Mnk2a isoform.  The sequence of the antigen has no homology with Mnk1 or Mnk2b.

The antibody is prepared from pooled rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Biological Significance: ExpandCollapse

MAP kinase-interacting kinases Mnk1 and Mnk2 are activated by the MAP kinases, ERK and p38 (Waskiewicz et al., 1997).  Additionally, Mnk1 and Mnk2 have been shown to phosphorylate the translation initiation factor eIF4E (Fukunaga and Hunter 1997; Waskiewicz et al., 1997).   Alternative splicing of the MKNK2 gene results in two isoforms with different C-termini: one that has a MAPK-binding domain, Mnk2a, and one that does not, Mnk2b (Parra et al., 2005).  Recent studies have indicated that Mnk2a has tumor suppressive activity while Mnk2b is pro-oncogenic (Maimon et al., 2014).

Synonyms: ExpandCollapse

• ADCAD1 antibody
• MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A) antibody
• MEF2 antibody
• MEF2A antibody
• MEF2A_HUMAN antibody
• Myocyte enhancer factor 2A antibody
• Myocyte-specific enhancer factor 2A antibody
• RSRFC4 antibody
• RSRFC9 antibody
• Serum response factor like protein 1 antibody
• Serum response factor-like protein 1 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Fukunaga, R. and Hunter, T., 1997. MNK1, a new MAP kinase‐activated protein kinase, isolated by a novel expression screening method for identifying protein kinase substrates. The EMBO journal, 16(8), pp.1921-1933. PMID: 9155018

Maimon, A., Mogilevsky, M., Shilo, A., Golan-Gerstl, R., Obiedat, A., Ben-Hur, V., Lebenthal-Loinger, I., Stein, I., Reich, R., Beenstock, J. and Zehorai, E., 2014. Mnk2 alternative splicing modulates the p38-MAPK pathway and impacts Ras-induced transformation. Cell Reports, 7(2), pp.501-513. PMID: 24726367

Parra, J.L., Buxadé, M. and Proud, C.G., 2005. Features of the catalytic domains and C termini of the MAPK signal-integrating kinases Mnk1 and Mnk2 determine their differing activities and regulatory properties. Journal of Biological Chemistry, 280(45), pp.37623-37633. PMID: 16162500

Waskiewicz, A.J., Flynn, A., Proud, C.G. and Cooper, J.A., 1997. Mitogen‐activated protein kinases activate the serine/threonine kinases Mnk1 and Mnk2. The EMBO journal, 16(8), pp.1909-1920. PMID: 9155017

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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