Affinity purified from pooled serum. Learn more.

Mnk2a Antibody

Catalog #: 1180-Mnk2a Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Human, Non-human primate
Applications:
WB 1:500
Species:
Rabbit Polyclonal
Molecular Reference:
~52 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1180-Mnk2a, RRID:AB_2631038
Antigen/Purification: ExpandCollapse

The antigen is a peptide from the C-terminal region, specific to the human Mnk2a isoform.  The sequence of the antigen has no homology with Mnk1 or Mnk2b.

The antibody is prepared from pooled rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Biological Significance: ExpandCollapse

MAP kinase-interacting kinases Mnk1 and Mnk2 are activated by the MAP kinases, ERK and p38 (Waskiewicz et al., 1997).  Additionally, Mnk1 and Mnk2 have been shown to phosphorylate the translation initiation factor eIF4E (Fukunaga and Hunter 1997; Waskiewicz et al., 1997).   Alternative splicing of the MKNK2 gene results in two isoforms with different C-termini: one that has a MAPK-binding domain, Mnk2a, and one that does not, Mnk2b (Parra et al., 2005).  Recent studies have indicated that Mnk2a has tumor suppressive activity while Mnk2b is pro-oncogenic (Maimon et al., 2014).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

General References

Fukunaga, R., & Hunter, T. (1997) A New MAP Kinase-Activated Protein Kinase Isolated by a Novel Expression Screening Method to Identify Protein Kinase Substrates. EMBO J. Apr 15;16(8):1921-33.

Maimon  A, Mogilevsky M, Shilo A, Golan-Gerstl R, Obiedat A, Ben-Hur V, Lebenthal-Loinger I, Stein I, Reich R, Beenstock J, Zehorai E, Andersen CL, Thorsen K, Orntoft TF, Davis RJ, Davidson B, Mu D, Karni R (2014) Mnk2 Alternative Splicing Modulates the p38-MAPK Pathway and Impacts Ras-Induced Transformation. Cell Reports 7, 501-513.

Parra JL, Buxadé M, Proud CG (2005) Features of the Catalytic Domains and C Termini of the MAPK Signal-integrating Kinases Mnk1 and Mnk2 Determine Their Differing Activities and Regulatory Properties. J. Biol. Chem. 280, 37623-37633.

Waskiewicz AJ, Flynn A, Proud CG, Cooper JA (1997) Mitogen-activated Protein Kinases Activate the Serine/Threonine Kinases Mnk1 and Mnk2. EMBO J. 16,1909-1920.

 

Mnk2a antibody
Western blot of HeLa lysate showing specific immunolabeling of the ~52 kDa Mnk2a isoform in the third lane (-). Specificity is shown in the second lane (Mnk2a) where immunolabeling is blocked by preadsorption of the Mnk2a peptide used as the antigen, but not by the Mnk2b peptide in the first lane.

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