Microtubule Associated Protein 2C/D Antibody

Catalog #: 1101-MAP2C Categories: , , Datasheet:

$109.00$365.00

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Excellent marker for studying neuronal development!

 

Formulation:
Protein G Purified
Species Tested:
Rat, Mouse, Human, Bovine
Applications:
WB 1:5000IHC 1:2500-1:10,000 ICC 1:2500-1:10,000
Species:
Mouse Monoclonal
Gene Name:
MAP2
Molecular Reference:
~ 70 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1101-MAP2C, RRID:AB_2571638
Antigen/Purification: ExpandCollapse

The antigen is a full length recombinant human MAP2D protein.

The antibody is Protein G purified.

Biological Significance: ExpandCollapse

Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994). MAP2 is made up of two ~280 kDa bands referred to as MAP2a and MAP2b. A third lower molecular weight form, MAP2C and MAP2D, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2C and MAP2D are expressed earlier in development than the MAP2a and MAP2b isoforms, so that this antibody is a more useful marker of neuronal development. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone (FontaineLenore V. et al., 2006).

Synonyms: ExpandCollapse

• DKFZp686I2148 antibody
• MAP 2 antibody
• MAP dendrite specific antibody
• MAP-2 antibody
• MAP2 antibody
• MAP2C antibody
• MAP2D antibody
• Microtubule associated protein 2 antibody
• Microtubule-associated protein 2 antibody
• MTAP2_HUMAN antibody

Storage

100 µl in PBS + 50% glycerol and 5 mM sodium azide.

For long term storage –20° C is recommended. Store at –20 ° C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.

Product Specific Protocols

Immunocytochemistry

Rat mixed neuron and glia cells

Immunohistochemistry

Rat hippocampus

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Kindler S, Gardner CC (1994) Four repeat MAP2 isoforms in human and rat brain. Brain Res Mol Brain Res. 26(1-2):218-224.PMID: 7854050

Fontaine-Lenore V, Chambraud B, Fellous A, David S, Duchossoy Y, Baulieu EE, Robel P (2006) Microtubule-associated protein 2 (MAP2) is a neurosteroid. Proc Natl Acad Sci USA 103(12):4711-6. PMID: 16537405

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Microtubule Associated Protein 2C/D Antibody
Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~70 kDa MAP2C/D proteins and the ~280 kDa MAP2A/B proteins.

Click here to view our Western blotting and lysate preparation protocols.
Microtubule Associated Protein 2C/D Antibody
Immunostaining of mixed neuron and glial cultures showing specific cytoplasmic labeling of dendrites and perikarya of neuronal cells with anti-microtubule associated protein 2C/D (cat #1101-MAP2C, red, 1:2500) and specific fibroblast, astrocyte labeling with anti-vimentin (cat #2105-VIM, green, 1:500). The blue stain is DAPI to identify nuclei.

Download the protocol used for this image:
Immunofluorescence of a section of adult rat hippocampus section stained with Anti-MAP2C (cat # 1101-MAP2C, green, 1:5,000) and an anti-FOX2 antibody (red). Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies. The anti-MAP2C labels all MAP2 protein isotypes expressed in neuronal perikarya and dendrites.

Download the protocol used for this image:

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