Microtubule Associated Protein 2 (MAP2) Antibody

Catalog #: 1100-MAP2 Categories: , , , Datasheet:

$99.00$310.00

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Great for staining neuronal dendrites!

 

Formulation:
Total IgY fraction
Species Tested:
Bovine, Human, Mouse, Rat
Expected Reactivity:
Most Mammals
Applications:
WB 1:20000IHC 1:2500-1:10,000ICC 1:1000-1:20,000
Species:
Chicken polyclonal
Gene Name:
MAP2
Molecular Weight:
~280 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1100-MAP2, RRID:AB_2492141
Antigen/Purification: ExpandCollapse

The antigen is a recombinant bovine MAP2 expressed in and purified from E. coli.

The antibody is total IgY fraction.

Biological Significance: ExpandCollapse

Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein ? (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994). MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein ? (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone (Fontaine-Lenore V. et al., 2006).

Synonyms: ExpandCollapse

• DKFZp686I2148 antibody
• MAP 2 antibody
• MAP dendrite specific antibody
• MAP-2 antibody
• MAP2 antibody
• MAP2A antibody
• MAP2B antibody
• MAP2C antibody
• Microtubule associated protein 2 antibody
• Microtubule-associated protein 2 antibody
• MTAP2_HUMAN antibody

Storage

100 µl total IgY fraction in PBS + 10 mM NaN3. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Immunocytochemistry

Rat mixed neuron and glial culture
E20 rat cortical neuron and glial culture

Immunohistochemistry

Rat brain stem

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunocytochemistry: Mouse Hippocampal Neurons


Immunocytochemistry: Mouse Hippocampal Neurons
PMID Dilution Publication
27762280 1:2000 Allen, M., et al. 2016. Protease induced plasticity: matrix metalloproteinase-1 promotes neurostructural changes through activation of protease activated receptor 1. Scientific Reports, 6.
24784359 1:20,000 Ho, V.M., et al. 2014. GluA2 mRNA distribution and regulation by miR-124 in hippocampal neurons. Molecular and Cellular Neuroscience , 61, pp.1-12.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Microtubule Associated Protein 2 (MAP2) Antibody
Western blot of rat cortical lysate showing specific immunolabeling of the ~280 kDa MAP2 protein.

Click here to view our Western blotting and lysate preparation protocols.

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