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Microtubule Associated Protein 2 (MAP2) Antibody

Chicken Polyclonal Antibody from Aves Labs.

Catalog #: MAP Categories: , , , Datasheet:

$399.00

Size:
1000 µl
Formulation:
Affinity Purified IgY
Species Tested:
Mouse
Expected Reactivity:
Human, Mouse, Rat
Applications:
WB 1:5000-1:10000IHC 1:1000-1:2000ICC 1:1000-1:2000
Host Species:
Chicken Polyclonal
Gene Name:
MAP2
Molecular Weight:
280 kDa
Cite This Antibody:
Aves Labs Cat# MAP, RRID:AB_2313549
Antigen/Purification: ExpandCollapse

Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the MAP-2 gene product, but are shared between the human (NP_002365, NCBI) and mouse (P20357, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 ug/ml. Finally, equal volumes of both of these affinitypurified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Synonyms: ExpandCollapse

• DKFZp686I2148 antibody
• MAP 2 antibody
• MAP dendrite specific antibody
• MAP-2 antibody
• MAP2 antibody
• MAP2A antibody
• MAP2B antibody
• MAP2C antibody
• Microtubule associated protein 2 antibody
• Microtubule-associated protein 2 antibody
• MTAP2_HUMAN antibody

Storage

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS,” pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

Product Specific References

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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