Our Anti-Microtubule Associated Protein 2 (MAP2) primary antibody from PhosphoSolutions is goat polyclonal. It detects human, mouse, and rat Microtubule Associated Protein 2 (MAP2) and is Protein G purified. It is great for use in WB, IHC, ICC.
Immunofluorescence of a section of rat brain stem showing specific labeling of MAP2 (cat.1099-MAP2, 1:2000, red) in the perikarya and dendrites of neurons and specific labeling of the myelin sheath around axons with anti-MBP ( cat.1120-MBP , 1:5000, green). The blue is DAPI revealing nuclear DNA.
Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994). MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein t (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone (Fontaine-Lenore V. et al., 2006).
Protein G Purified
Polyclonal
IgG
ICC, IHC, WB
Goat
MAP2
280 kDa
Recombinant human MAP2 protein expressed in and purified from E. Coli.
Human, Mouse, Rat
AB_2752241
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Specific for endogenous levels of the ~280 kDa MAP2 protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
DKFZp686I2148 antibody, MAP 2 antibody, MAP dendrite specific antibody, MAP-2 antibody, MAP2 antibody, MAP2A antibody, MAP2B antibody, MAP2C antibody, Microtubule associated protein 2 antibody, Microtubule-associated protein 2 antibody, MTAP2_HUMAN antibody
Fontaine-Lenore V, Chambraud B, Fellous A, David S, Duchossoy Y, Baulieu EE, Robel P (2006) Microtubule-associated protein 2 (MAP2) is a neurosteroid. Proc Natl Acad Sci USA 103(12):4711-6. PMID: 16537405
Kindler S, Gardner CC (1994) Four repeat MAP2 isoforms in human and rat brain. Brain Res Mol Brain Res. 26(1-2):218-224. PMID: 7854050
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