Microtubule Associated Protein 2 (MAP2) Antibody

Catalog #: 1099-MAP2 Categories: , Datasheet:

$109.00$365.00

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New goat MAP2 is an excellent dendritic marker especially useful in double labeling experiments!

Formulation:
Protein G purified
Species Tested:
Human, Mouse, Rat
Applications:
WB 1:1000IHC 1:1000-1:2000ICC 1:1000-1:2000
Species:
Goat Polyclonal
Gene Name:
MAP2
Molecular Reference:
~280 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1099-MAP2: RRID:AB_2752241
Antigen/Purification: ExpandCollapse

The antigen is recombinant human MAP2 expressed in and purified from E. coli.

The antibody is Protein G purified.

Biological Significance: ExpandCollapse

Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994). MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein t (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone (Fontaine-Lenore V. et al., 2006).

Synonyms: ExpandCollapse

• DKFZp686I2148 antibody
• MAP 2 antibody
• MAP dendrite specific antibody
• MAP-2 antibody
• MAP2 antibody
• MAP2A antibody
• MAP2B antibody
• MAP2C antibody
• Microtubule associated protein 2 antibody
• Microtubule-associated protein 2 antibody
• MTAP2_HUMAN antibody

Storage

100 µl in PBS + 50% glycerol + 5 mM NaN3. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunohistochemistry

Rat Brain Stem

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Fontaine-Lenore V, Chambraud B, Fellous A, David S, Duchossoy Y, Baulieu EE, Robel P (2006) Microtubule-associated protein 2 (MAP2) is a neurosteroid. Proc Natl Acad Sci USA 103(12):4711-6. PMID: 16537405

Kindler S, Gardner CC (1994) Four repeat MAP2 isoforms in human and rat brain. Brain Res Mol Brain Res. 26(1-2):218-224. PMID: 7854050

 

 

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Western blot of rat cortical lysate showing specific immunolabeling of the ~280 kDa MAP2 protein.

Click here to view our Western blotting and lysate preparation protocols.
Immunofluorescence of a section of rat brain stem showing specific labeling of MAP2 (cat #1099-MAP2, 1:2000, red) in the perikarya and dendrites of neurons and specific labeling of the myelin sheath around axons with anti-MBP (cat #1120-MBP, 1:5000, green). The blue is DAPI revealing nuclear DNA.

Download the protocol used for this image: