Microtubule Associated Protein 2 (MAP2) Antibody

Catalog #: 1099-MAP2 Categories: , Datasheet:

$109.00$365.00

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New goat MAP2 is an excellent dendritic marker especially useful in double labeling experiments!

Formulation:
Protein G purified
Species Reactivity:
Human, Mouse, Rat
Applications:
WB 1:1000IF 1:500
Species:
Goat
Gene Name:
MAP2
Molecular Reference:
~280 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1099-MAP2: RRID:AB_2752241
Antigen/Purification: ExpandCollapse

The antigen is recombinant human MAP2 expressed in and purified from E. coli.

The antibody is Protein G purified.

Biological Significance: ExpandCollapse

Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994). MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein t (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone (Fontaine-Lenore V. et al., 2006).

Synonyms: ExpandCollapse

• DKFZp686I2148 antibody
• MAP 2 antibody
• MAP dendrite specific antibody
• MAP-2 antibody
• MAP2 antibody
• MAP2A antibody
• MAP2B antibody
• MAP2C antibody
• Microtubule associated protein 2 antibody
• Microtubule-associated protein 2 antibody
• MTAP2_HUMAN antibody

Storage

Liquid containing 10mM sodium azide.

For long term storage –20° C is recommended in undiluted aliquots. Stable at –20° C for at least 1 year. Avoid freeze/thaw cycles.

General References:

Fontaine-Lenore V, Chambraud B, Fellous A, David S, Duchossoy Y, Baulieu EE, Robel P (2006) Microtubule-associated protein 2 (MAP2) is a neurosteroid. Proc Natl Acad Sci USA 103(12):4711-6.

Kindler S, Gardner CC (1994) Four repeat MAP2 isoforms in human and rat brain. Brain Res   Mol Brain Res. 26(1-2):218-224.

 

 

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Western blot of rat cortical lysate showing specific immunolabeling of the ~280 kDa MAP2 protein.
Immunostaining of rat brain stem showing specific labeling of MAP2 in the perikarya and dendrites of neurons in red and MBP in green (cat.# 1120-MBP). Cell nuclei are visualized with DAPI DNA stain.