MeCP2 (Methyl-CpG Binding Protein 2) Antibody

$109.00$365.00

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Nuclear neuronal marker for use in IHC!

Formulation:
Protein G purified
Specificity:
Human, Mouse, Rat
Applications:
WB 1:2,000IF 1:1,000IHC 1:1,000
Species:
Mouse Monoclonal
Gene Name:
MECP2
Molecular Reference:
~75 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1205-MeCP2, RRID:AB_2632401
Antigen/Purification: ExpandCollapse

The antigen is recombinant full length human MeCP2 expressed in and purified from E. Coli.

The antibody is protein G purified from culture supernatant.

Biological Significance: ExpandCollapse

MeCP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA.  It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs.  MeCP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A (Nan et al., 1998).  Defects in MeCP2 are the cause of Rett syndrome (RTT) (Amir et al., 1999).  RTT is an X-linked dominant disease; it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.

Storage

100 µl in PBS + 50% glycerol +5 mM sodium azide.

For long term storage –20° C is recommended in undiluted aliquots. Stable at –20° C for at least 1 year. Avoid freeze/thaw cycles.

General References

Amir RE,  Van den Veyver IB ,  Wan M ,  Tran CQ ,  Francke U ,  Zoghbi HY  (1999) Rett syndrome is caused by mutations in X-linked MECP2, encoding methyl-CpG-binding protein 2.  Nat Genet.  Oct;23(2):185-8.

Nan X ,  Ng HH ,  Johnson CA ,  Laherty CD ,  Turner BM ,  Eisenman RN ,  Bird A  (1998) Transcriptional repression by the methyl-CpG-binding protein MeCP2 involves a histone deacetylase complex.  Nature.  May 28;393(6683):386-9.

MeCP2 antibody
Western blot of mouse whole brain lysate showing specific immunolabeling of the MeCP2 protein at ~75 kDa.
Methyl-CpG Binding Protein 2 (MeCP2) Antibody
Rat hippocampal section showing specific nuclear MeCP2 immunolabeling in red and FOX3 in green.