MeCP2 (Methyl-CpG Binding Protein 2) Antibody

Catalog #: 1205-MeCP2 Categories: , , , Datasheet:

$109.00$365.00

  • SizePrice
Clear

Nuclear neuronal marker for use in IHC!

Formulation:
Protein G purified
Species Tested:
Human, Mouse, Rat
Applications:
WB 1:2000IHC 1:500-1:5000ICC 1:1000-1:5000
Species:
Mouse Monoclonal
Gene Name:
MECP2
Molecular Reference:
~75 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1205-MeCP2, RRID:AB_2632401
Antigen/Purification: ExpandCollapse

The antigen is recombinant full length human MeCP2 expressed in and purified from E. Coli.

The antibody is protein G purified from culture supernatant.

Biological Significance: ExpandCollapse

MeCP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA.  It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs.  MeCP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A (Nan et al., 1998).  Defects in MeCP2 are the cause of Rett syndrome (RTT) (Amir et al., 1999).  RTT is an X-linked dominant disease; it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.

Synonyms: ExpandCollapse

• AUTSX 3 antibody
• AUTSX3 antibody
• DKFZp686A24160 antibody
• Mbd 5 antibody
• Mbd5 antibody
• MECP 2 antibody
• MeCP 2 protein antibody
• MeCP-2 protein antibody
• Mecp2 antibody
• MECP2_HUMAN antibody
• Methyl CpG binding protein 2 (Rett syndrome) antibody
• Methyl CpG binding protein 2 antibody
• Methyl-CpG-binding protein 2 antibody
• MRX 16 antibody
• MRX 79 antibody
• MRX16 antibody
• MRX79 antibody
• MRXS 13 antibody
• MRXS13 antibody
• MRXSL antibody
• PPMX antibody
• RS antibody
• RTS antibody
• RTT antibody
• WBP 10 antibody
• WBP10 antibody

Storage

100 µl in PBS + 50% glycerol +5 mM sodium azide.

For long term storage –20° C is recommended in undiluted aliquots. Stable at –20° C for at least 1 year. Avoid freeze/thaw cycles.

Product Specific Protocols

Immunohistochemistry

Rat cerebellum, free floating sections colabeled with Anti-GFAP (cat# 620-GFAP)
Rat cerebellum, free floating sections colabeled with Anti-GFAP (cat# 621-GFAP)
Rat hippocampus, colabeled with anti-adenylate cyclase III (cat#85-AC3)
Rat hippocampus

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Amir, R.E., Van den Veyver, I.B., Wan, M., Tran, C.Q., Francke, U. and Zoghbi, H.Y., 1999. Rett syndrome is caused by mutations in X-linked MECP2, encoding methyl-CpG-binding protein 2. Nature genetics, 23(2), p.185. PMID: 10508514

Nan, X., Ng, H.H., Johnson, C.A., Laherty, C.D., Turner, B.M., Eisenman, R.N. and Bird, A., 1998. Transcriptional repression by the methyl-CpG-binding protein MeCP2 involves a histone deacetylase complex. Nature, 393(6683), p.386. PMID: 9620804

Reviews

There are no reviews yet.

Be the first to review “MeCP2 (Methyl-CpG Binding Protein 2) Antibody”

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

MeCP2 antibody
Western blot of mouse whole brain lysate showing specific immunolabeling of the MeCP2 protein at ~75 kDa.

Click here to view our Western blotting and lysate preparation protocols.

You may also like…