Metabotropic Glutamate Receptor 5/1a Antibody

Catalog #: 2032-mGluR5/1 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Formulation:
Affinity purified
Species Reactivity:
Rat, Mouse, Human
Applications:
WB 1:1000IHC 1:500
Species:
Rabbit
Gene Name:
GRM5
Molecular Weight:
~125 kDa and ~250 kDa
Cite This Antibody:
PhosphoSolutions Cat# 2032-mGluR5/1, RRID:AB_2492152
Antigen/Purification: ExpandCollapse

The antigen is a peptide from the C-terminal region of rat mGluR5 and rat mGluR1a.

The antibody is prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Biological Significance: ExpandCollapse

The metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse (Bhattacharya et al., 2004; Francesconi et al., 2004; Wilson and Nicoll, 2001). Group I metabotropic glutamate receptors (consisting of mGluR1 and mGluR5) are G-protein-coupled neurotransmitter receptors that are localized in the perisynaptic region of the postsynaptic membrane. When activated, Group I mGluRs lead to stimulation of phospholipase and activation of Protein Kinase C. In contrast, activation of Group II metabotropic receptors (mGluR2 and mGluR3) leads to inhibition of adenylate cyclase. The mGluR1 receptor may also be critically involved in limiting the deleterious effects of excitotoxicity (Blaabjerg et al., 2003). In contrast, the mGluR5 receptor appears to be essential for late phase LTP in area CA1 of the hippocampus (Francesconi et al., 2004).

Synonyms: ExpandCollapse

• glutamate receptor, metabotropic 5
• GPRC1EMGLUR5metabotropic glutamate receptor 5
• mGlu5
• mGluR5

Storage

100 µl in 200 mM Tris-Glycine (pH 7.5), 0.1% sodium azide. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific References

Bellini, S., Fleming, K.E., De, M., McCauley, J.P., Petroccione, M.A., D’Brant, L.Y., Tkachenko, A., Kwon, S., Jones, L.A. and Scimemi, A., 2018. Neuronal glutamate transporters control dopaminergic signaling and compulsive behaviors. J Neurosci., 38(4), pp.937-961. PMID: 29229708

 

 

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Metabotropic Glutamate Receptor 5/1a Antibody
Western blot of 10 µg of HEK 293 cells expressing: 1) mGluR5 and 2) mGluR1a showing the specific immunolabeling of the ~125 kDa monomer and the ~250 kDa dimers of both mGluR1a (2) and mGluR5 (1).

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