Metabotropic Glutamate Receptor 5/1a Antibody

Our mGluR 5/1a rabbit polyclonal antibody is affinity purified. Optimized in WB and IHC.

Catalog #: 2032-mGluR5/1 Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Formulation:
Affinity purified
Species Tested:
Mouse, Rat
Expected Reactivity:
Human
Applications:
WB 1:1000IHC 1:500 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
GRM5
Molecular Weight:
~125 kDa and ~250 kDa
Cite This Antibody:
PhosphoSolutions Cat# 2032-mGluR5/1, RRID:AB_2492152
Antigen/Purification: ExpandCollapse

The antigen is a peptide from the C-terminal region of rat mGluR5 and rat mGluR1a.

The antibody is prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Biological Significance: ExpandCollapse

The metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse (Bhattacharya et al., 2004; Francesconi et al., 2004; Wilson and Nicoll, 2001). Group I metabotropic glutamate receptors (consisting of mGluR1 and mGluR5) are G-protein-coupled neurotransmitter receptors that are localized in the perisynaptic region of the postsynaptic membrane. When activated, Group I mGluRs lead to stimulation of phospholipase and activation of Protein Kinase C. In contrast, activation of Group II metabotropic receptors (mGluR2 and mGluR3) leads to inhibition of adenylate cyclase. The mGluR1 receptor may also be critically involved in limiting the deleterious effects of excitotoxicity (Blaabjerg et al., 2003). In contrast, the mGluR5 receptor appears to be essential for late phase LTP in area CA1 of the hippocampus (Francesconi et al., 2004).

Synonyms: ExpandCollapse

• glutamate receptor, metabotropic 5
• GPRC1EMGLUR5metabotropic glutamate receptor 5
• mGlu5
• mGluR5

Storage

100 µl in 200 mM Tris-Glycine (pH 7.5), 0.1% sodium azide. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Mouse


Western Blot: Mouse
PMID Dilution Publication
29229708 1:2000 Bellini, S., et al. 2018 Neuronal glutamate transporters control dopaminergic signaling and compulsive behaviors. J Neurosci., 38(4), pp.937-961.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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