Metabotropic Glutamate Receptor 2/3 Antibody

Catalog #: 2030-mGluR2/3 Categories: , Datasheet:

$109.00$365.00

  • SizePrice
Clear

Rabbit polyclonal antibody

Formulation:
Affinity purified
Species Tested:
Rat
Expected Reactivity:
Bovine, Canine, Human, Mouse, Non-human primate, Zebra fish
Applications:
WB 1:1000IHC 1:500 Don't see your application?
Species:
Rabbit
Gene Name:
GRM2/3
Molecular Weight:
~110 kDa and ~220 kDa
Cite This Antibody:
PhosphoSolutions Cat# 2030-mGluR2/3, RRID:AB_2492151
Antigen/Purification: ExpandCollapse

The antigen is a peptide from the C-terminal region of rat mGluR2 and rat mGluR3.

The antibody is prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Biological Significance: ExpandCollapse

The metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse (Bhattacharya et al., 2004; Francesconi et al., 2004; Wilson and Nicoll, 2001). Group I metabotropic glutamate receptors (consisting of mGluR1 and mGluR5) are G-protein-coupled neurotransmitter receptors that are localized in the perisynaptic region of the postsynaptic membrane. When activated, Group I mGluRs lead to stimulation of phospholipase and activation of Protein Kinase C. In contrast, activation of Group II metabotropic receptors (mGluR2 and mGluR3) leads to inhibition of adenylate cyclase. The mGluR2 subunit has been shown to be required for long-term potentiation at the mossy fiber input in the hippocampus (Yokoi et al., 1996).

Synonyms: ExpandCollapse

• GLUR2 antibody
• GLUR3 antibody
• Glutamate receptor homolog antibody
• Glutamate receptor metabotropic 2 antibody
• Glutamate receptor metabotropic 3 antibody
• GPRC1B antibody
• GPRC1C antibody
• GRM2 antibody
• GRM3 antibody
• Metabotropic glutamate receptor 2 antibody
• Metabotropic glutamate receptor 3 antibody
• mGlu2 antibody
• mGlu3 antibody
• MGLUR2 antibody
• MGLUR3 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Bhattacharya M, Babwah AV, Godin C, Anborgh PH, Dale LB, Poulter MO, Ferguson SSG (2004) Ral and phospholipase D2-dependent pathway for constitutive metabotropic glutamate receptor endocytosis. J Neurosci, 24:8752-8761. PMID: 15470141

Francesconi W, Cammalleri M, Sanna PP (2004) The metabotropic glutamate receptor 5 is necessary for late-phase long-term potentiation in the hippocampal CA1 region. Brain Res, 1022:12-18. PMID: 15353208

Wilson RI, Nicoll RA (2001) Endogenous cannabinoids mediate retrograde signalling at hippocampal synapses. Nature (London), 410:588-592. PMID: 11279497

Yokoi M, Kobayashi K, Manabe T, Takahashi T, Sakaguchi I, Katsuura G, Shigemoto R, Ohishi H, Nomura S, Nakamura K, Nakao K, Katsuki M, Nakanishi S (1996) Impairment of hippocampal mossy fiber LTD in mice lacking mGluR2. Science, 273:645-647. PMID: 8662555

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
Leave a Reply

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

Metabotropic Glutamate Receptor 2/3 Antibody
Western blot of 10 µg of rat hippocampal lysate showing the specific immunolabeling of the ~110 kDa monomer and the ~220 kDa dimer of mGluR2 and mGluR3.

Click here to view our Western blotting and lysate preparation protocols.

You may also like…