Affinity purified from pooled serum. Learn more.

MEK5 (Ser311 Thr315) Antibody

Catalog #: p183-3115 Category: Datasheet:


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Rabbit polyclonal antibody

Pooled Serum
Affinity Purified from Pooled Serum
Species Tested:
Species Reactivity:
Mouse, Human, Bovine, Canine, Chicken, Non-human primate, Xenopus, Zebra fish
WB 1:1000 Don't see your application?
Gene Name:
Molecular Weight:
~49 kDa
Cite This Antibody:
PhosphoSolutions Cat# p183-3115, RRID:AB_2492149
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser311, Thr315 of MEK5.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

MEK5 (also known as MKK5) is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family. MEK5 has been shown to specifically activate ERK5 (Zhou et al., 1995) whereas MEK5 itself is regulated by MEKK3 (Chao et al., 1999). An important link between MEK5 and metastatic prostrate cancer has been demonstrated (Mehta, 2003). Dual phosphorylation of Ser311 and Thr315 have been implicated in cell proliferation (Cameron et al., 2004).

Synonyms: ExpandCollapse

• Dual specificity mitogen activated protein kinase kinase 5 antibody
• Dual specificity mitogen-activated protein kinase kinase 5 antibody
• EC antibody
• HsT17454 antibody
• MAP kinase kinase 5 antibody
• MAP kinase kinase MEK5b antibody
• MAP2K5 antibody
• MAPK/ERK kinase 5 antibody
• MAPKK 5 antibody
• MAPKK5 antibody
• MEK 5 antibody
• mitogen-activated protein kinase kinase 5 antibody
• MKK5 antibody
• MP2K5_HUMAN antibody
• PRKMK5 antibody
• Protein kinase, mitogen-activated, kinase 5 antibody
• SAPKK5 antibody
• SKK5 antibody


100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Zhou G, Bao ZQ, Dixon JE, (1995) Components of a new human protein kinase signal transduction pathway. J. Biol. Chem. 270(21):12665-9. PMID: 7759517

Chao TH, Hayashi M, Tapping RI, Kato Y, Lee JD (1999) MEKK3 directly regulates MEK5 activity as part of the big mitogen-activated protein kinase 1 (BMK1) signaling pathway. J. Biol. Chem. 274(51):36035-8. PMID: 10593883

Mehta PB, Jenkins BL, McCarthy L, Thilak L, Robson CN, Neal DE, Leung HY (2003) MEK5 overexpression is associated with metastatic prostate cancer, and stimulates proliferation, MMP-9 expression and invasion. Oncogene 22(9):1381-9. PMID: 12618764

Cameron SJ, Abe J, Malik S, Che W, Yang J (2004) Differential role of MEK5alpha and MEK5beta in BMK1/ERK5 activation. J. Biol. Chem. 279(2):1506-12. PMID: 14583600

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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MEK5 ser311, thr315 Antibody
Western blot of rat testes lysate showing specific labeling of the ~49 kDa MEK5 protein phosphorylated at Ser311 Thr315 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Click here to view our Western blotting and lysate preparation protocols.

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