Affinity purified from pooled serum. Learn more.

MEK1/2 (Ser218,222) Antibody

Rabbit polyclonal antibody

Catalog #: p180-21822 Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse
Expected Reactivity:
Bovine, Canine, Chicken, Non-human primate, Rat, Xenopus, Zebra fish
Applications:
WB 1:1000 Don't see your application?
Host Species:
Rabbit
Gene Name:
MAP2K1/2
Molecular Weight:
~45 kDa
Cite This Antibody:
PhosphoSolutions Cat# p180-21822, RRID:AB_2492146
Antigen/Purification: ExpandCollapse

The antigen is a Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser218,222 of human MEK 1/2. Note: The phosphosites in human MEK 1/2 are Ser218 and Ser222; the phosphosites in mouse MEK 1/2 are Ser217 and Ser221.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in the brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992).

Synonyms: ExpandCollapse

• AA589381 antibody
• CFC3 antibody
• Dual specificity mitogen-activated protein kinase kinase 1 antibody
• Dual specificity mitogen-activated protein kinase kinase 2 antibody
• EC 2.7.12.2 antibody
• ERK activator kinase 1 antibody
• ERK activator kinase 2 antibody
• FLJ26075 antibody
• MAP kinase kinase 1 antibody
• MAP kinase kinase 2 antibody
• MAP2K1 antibody
• MAP2K2 antibody
• MAPK/ERK kinase 1 antibody
• MAPK/ERK kinase 2 antibody
• MAPKK 1 antibody
• MAPKK1 antibody
• MAPKK2 antibody
• MEK 1 antibody
• MEK1 antibody
• MEKK1 antibody
• Mitogen activated protein kinase kinase 1 antibody
• Mitogen activated protein kinase kinase 2 antibody
• Mitogen-activated protein kinase kinase 2, p45 antibody
• MK2 antibody
• MKK 1 antibody
• MKK 2 antibody
• MKK1 antibody
• MKK2 antibody
• MP2K1_HUMAN antibody
• PRKMK 1 antibody
• PRKMK 2 antibody
• Prkmk1 antibody
• Prkmk2 antibody
• protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) antibody
• Protein kinase, mitogen-activated, kinase 1 antibody
• Protein kinase, mitogen-activated, kinase 2 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Adams JP, Sweatt JD (2002) Molecular psychology: Roles for the ERK MAP kinase cascade in memory. Annual Review of Pharmacology and Toxicology 42:135-163. PMID: 10508514

Ahn NG (1993) The MAP kinase cascade. Discovery of a new signal transduction pathway. Molecular and Cellular Biochemistry 127-128:201-209. PMID: 23055506

Chong H, Vikis HG, Guan KL (2003) Mechanisms of regulating the Raf kinase family. Cellular Signalling 15:463-469. PMID: 9620804

Crews CM, Alessandrini A, Erikson RL (1992) The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product. Science 258:478-480.PMID: 9620804

Kyriakis JM, Brautigan DL, Ingebritsen TS, Avruch J (1991) pp54 Microtubule-associated protein-2 kinase requires both tyrosine and serine/threonine phosphorylation for activity. Journal of Biological Chemistry 266:10043-10046.PMID: 9620804

Seger R, Ahn NG, Boulton TG, Yancopoulos GD, Panayotatos N, Radziejewska E, Ericsson L, Bratlien RL, Cobb MH, Krebs EG (1991) Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophos-phorylation on both tyrosine and threonine residues: Implications for their mechanism of activation. PNAS USA 88:6142-6146.PMID: 9620804

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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