Affinity purified from pooled serum. Learn more.

MEK1 (Thr386) Antibody

We are the original manufacturer of our MEK1 (Thr386) rabbit polyclonal phosphospecific antibody, which is affinity purified from pooled serum. Optimized in WB.

Catalog #: p180-386 Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Rat
Expected Reactivity:
Bovine, Canine, Chicken, Mouse, Non-human primate, Xenopus
Applications:
WB 1:1000 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
MAP2K1
Molecular Weight:
~45 kDa
Cite This Antibody:
PhosphoSolutions Cat# p180-386, RRID:AB_2492148
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr386 of human MEK 1.

The antibody is  prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in the brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992). Conversely, there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386 (Mansour et al., 1994).

Synonyms: ExpandCollapse

• Dual specificity mitogen activated protein kinase kinase 1 antibody
• Dual specificity mitogen-activated protein kinase kinase 1 antibody
• ERK activator kinase 1 antibody
• MAP kinase kinase 1 antibody
• MAP kinase/Erk kinase 1 antibody
• MAP2K1 antibody
• MAPK/ERK kinase 1 antibody
• MAPKK 1 antibody
• MAPKK1 antibody
• MEK 1 antibody
• Mek1 antibody
• MEKK1 antibody
• Mitogen activated protein kinase kinase 1 antibody
• MKK 1 antibody
• MKK1 antibody
• MP2K1_HUMAN antibody
• PRKMK1 antibody
• Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody
• Protein kinase mitogen activated, kinase 1 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Adams JP, Sweatt JD (2002) Molecular psychology: Roles for the ERK MAP kinase cascade in memory. Annual Review of Pharmacology and Toxicology 42:135-163. PMID: 10508514

Ahn NG (1993) The MAP kinase cascade. Discovery of a new signal transduction pathway. Molecualar and Cellular Biochemistry 127-128:201-209. PMID: 23055506

Chong H, Vikis HG, Guan KL (2003) Mechanisms of regulating the Raf kinase family. Cellular Signalling 15:463-469. PMID: 9620804

Crews CM, Alessandrini A, Erikson RL (1992) The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product. Science 258:478-480.PMID: 9620804

Kyriakis JM, Brautigan DL, Ingebritsen TS, Avruch J (1991) pp54 Microtubule-associated protein-2 kinase requires both tyrosine and serine/threonine phosphorylation for activity. Journal of Biological Chemistry 266:10043-10046.PMID: 9620804

Seger R, Ahn NG, Boulton TG, Yancopoulos GD, Panayotatos N, Radziejewska E, Ericsson L, Bratlien RL, Cobb MH, Krebs EG (1991) Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophos-phorylation on both tyrosine and threonine residues: Implications for their mechanism of activation. PNAS USA 88:6142-6146.PMID: 9620804

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
Leave a Reply

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

You may also like…