Affinity purified from pooled serum. Learn more.

MEK1 (Thr292) Antibody

We are the original manufacturer of our MEK1 (Thr292) rabbit polyclonal phosphospecific antibody, which is affinity purified from pooled serum. Optimized in WB.

Catalog #: p180-292 Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse
Expected Reactivity:
Bovine, Canine, Non-human primate, Rat
Applications:
WB 1:1000 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
MAP2K1
Molecular Weight:
~45 kDa
Cite This Antibody:
PhosphoSolutions Cat# p180-292, RRID:AB_2492147
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr292 of human MEK 1.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

MEK 1 (MAP kinase kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in the brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992). Conversely, there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386 (Mansour et al., 1994).

Synonyms: ExpandCollapse

• Dual specificity mitogen activated protein kinase kinase 1 antibody
• Dual specificity mitogen-activated protein kinase kinase 1 antibody
• ERK activator kinase 1 antibody
• MAP kinase kinase 1 antibody
• MAP kinase/Erk kinase 1 antibody
• MAP2K1 antibody
• MAPK/ERK kinase 1 antibody
• MAPKK 1 antibody
• MAPKK1 antibody
• MEK 1 antibody
• Mek1 antibody
• MEKK1 antibody
• Mitogen activated protein kinase kinase 1 antibody
• MKK 1 antibody
• MKK1 antibody
• MP2K1_HUMAN antibody
• PRKMK1 antibody
• Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody
• Protein kinase mitogen activated, kinase 1 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Mouse


Western Blot: Mouse
PMID Dilution Publication
25971971 not listed Tassin, T.C., et al. 2015 Tassin, T. C., Benavides, D. R., Plattner, F., Nishi, A., & Bibb, J. A. (2015). Regulation of ERK Kinase by MEK1 Kinase Inhibition in the Brain. Journal of Biological Chemistry, Jun 26;290(26):16319-29.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
Leave a Reply

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

You may also like…