Affinity purified from pooled serum. Learn more.

MEK1 (Thr292) Antibody

Catalog #: p180-292 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse
Species Reactivity:
Bovine, Canine, Non-human primate, Rat
Applications:
WB 1:1000 Don't see your application?
Species:
Rabbit
Gene Name:
MAP2K1
Molecular Weight:
~45 kDa
Cite This Antibody:
PhosphoSolutions Cat# p180-292, RRID:AB_2492147
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr292 of human MEK 1.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

MEK 1 (MAP kinase kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in the brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992). Conversely, there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386 (Mansour et al., 1994).

Synonyms: ExpandCollapse

• Dual specificity mitogen activated protein kinase kinase 1 antibody
• Dual specificity mitogen-activated protein kinase kinase 1 antibody
• ERK activator kinase 1 antibody
• MAP kinase kinase 1 antibody
• MAP kinase/Erk kinase 1 antibody
• MAP2K1 antibody
• MAPK/ERK kinase 1 antibody
• MAPKK 1 antibody
• MAPKK1 antibody
• MEK 1 antibody
• Mek1 antibody
• MEKK1 antibody
• Mitogen activated protein kinase kinase 1 antibody
• MKK 1 antibody
• MKK1 antibody
• MP2K1_HUMAN antibody
• PRKMK1 antibody
• Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody
• Protein kinase mitogen activated, kinase 1 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Mouse


Western Blot: Mouse
PMID Dilution Publication
25971971 not listed Tassin, T.C., et al. 2015 Tassin, T. C., Benavides, D. R., Plattner, F., Nishi, A., & Bibb, J. A. (2015). Regulation of ERK Kinase by MEK1 Kinase Inhibition in the Brain. Journal of Biological Chemistry, Jun 26;290(26):16319-29.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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MEK1 thr292 Antibody
Western blot of recombinant wild type MEK 1 (WT) and mutant MEK 1 (T292A) (Mut). Specific immunolabeling of the ~45 kDa MEK-1 protein phosphorylated at Thr292 is shown in the second lane where MAP kinase was coexpressed.

Click here to view our Western blotting and lysate preparation protocols.

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