Affinity purified from pooled serum. Learn more.

MEF2C (Ser222) Antibody

Catalog #: p1208-222 Categories: , Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human
Species Reactivity:
Bovine, Chicken, Guinea Pig, Mouse, Non-human primate, Rat, Sheep
Applications:
WB 1:1000 Don't see your application?
Species:
Rabbit Polyclonal
Molecular Weight:
~ 51 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1208-222, RRID:AB_2572427
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho Ser222 of the human MEF2C protein.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Biological Significance: ExpandCollapse

MEF2C, also known as MADS box transcription enhancer factor 2, polypeptide C, is one of 4 MEF2 (myocyte enhancer factor 2) transcription factors that encode proteins for development of skeletal muscle and brain proliferation and differentiation (McDermott et al, 1993), along with regulating stress-response during cardiac hypertrophy in mammals (Wu et al, 2015). Phosphorylation of MEF2C at serine 59 has been shown to be negatively regulated by integrin-linked kinase (ILK) (Dong et al, 2015). Recently, high MEF2C expression has been associated with a subset of acute myeloid leukemia (AML) patients with adverse-risk disease features and poor outcomes (Laszlo et al, 2015). Phosphorylation of MEF2C at serine 222 may play a key role in MEF2C signaling.

Synonyms: ExpandCollapse

• C5DELq14.3 antibody
• DEL5q14.3 antibody
• MADS box transcription enhancer factor 2 polypeptide C (myocyte enhancer factor 2C) antibody
• MADS box transcription enhancer factor 2, polypeptide C antibody
• MEF2C antibody
• MEF2C_HUMAN antibody
• Myocyte enhancer factor 2C antibody
• Myocyte specific enhancer factor 2C antibody
• Myocyte-specific enhancer factor 2C antibody
• OTTHUMP00000222409 antibody
• Similar to MADS box transcription enhancer factor 2 polypeptide C antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Mouse


Western Blot: Mouse
PMID Dilution Publication
29431698 1:1000 Brown, F.C, et al. 2018 MEF2C phosphorylation is required for chemotherapy resistance in acute myeloid leukemia. Cancer discovery, 8(4), pp.478-497.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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MEF2C Ser222 Antibody
Western blot of OCIAML2 lysate showing specific immunolabeling of the ~51 kDa MEF2C phosphorylated at Ser222 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase ((λ-Ptase, 1200 units for 30 minutes).

Click here to view our Western blotting and lysate preparation protocols.

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