MeCP2 (Ser80) Antibody

Catalog #: p1205-80 Categories: , Datasheet:

$99.00$310.00

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Rabbit polyclonal antibody

Formulation:
Serum
Species Tested:
Rat, Mouse, Human
Applications:
WB 1:1000IHC 1:100-1:250 Don't see your application?
Species:
Rabbit
Gene Name:
MECP2
Molecular Weight:
~75 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1205-80, RRID:AB_2492144
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser80 of MECP2 (Methyl-CpG Binding Protein 2) protein.

The antibody is serum.

Biological Significance: ExpandCollapse

MECP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MECP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Defects in MECP2 are the cause of Rett syndrome (RTT). RTT is an X-linked dominant disease, it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Recent studies have reported a new phosphorylation site at Ser80. Phosphorylation and dephosphorylation of this site may be involved in modulating the dynamic function of MECP2 in neurons transiting between resting and active states within neural circuits that underlie behaviors. (Tao et al., 2009)

Synonyms: ExpandCollapse

• AUTSX 3 antibody
• AUTSX3 antibody
• DKFZp686A24160 antibody
• Mbd 5 antibody
• Mbd5 antibody
• MECP 2 antibody
• MeCP 2 protein antibody
• MeCP-2 protein antibody
• Mecp2 antibody
• MECP2_HUMAN antibody
• Methyl CpG binding protein 2 (Rett syndrome) antibody
• Methyl CpG binding protein 2 antibody
• Methyl-CpG-binding protein 2 antibody
• MRX 16 antibody
• MRX 79 antibody
• MRX16 antibody
• MRX79 antibody
• MRXS 13 antibody
• MRXS13 antibody
• MRXSL antibody
• PPMX antibody
• RS antibody
• RTS antibody
• RTT antibody
• WBP 10 antibody
• WBP10 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended in undiluted aliquots. Stable at –20° C for at least 1 year after date of receipt. Avoid freeze/thaw cycles.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunohistochemistry: Human | Mouse
Western Blot: Human | Mouse


Immunohistochemistry: Human
PMID Dilution Publication
28367114 1:250 Maphis, N.M., et al. 2017 Whole Genome Expression Analysis in a Mouse Model of Tauopathy Identifies MECP2 as a Possible Regulator of Tau Pathology. Frontiers in Molecular Neuroscience, 10:69.


Immunohistochemistry: Mouse
PMID Dilution Publication
28367114 1:1000 Maphis, N.M., et al. 2017 Whole Genome Expression Analysis in a Mouse Model of Tauopathy Identifies MECP2 as a Possible Regulator of Tau Pathology. Frontiers in Molecular Neuroscience, 10:69.
19225110 not listed Tao, J., et al. 2009 Phosphorylation of MeCP2 at Serine 80 regulates its chromatin association and neurological function. Proc Natl Acad Sci U S A 106(12):4882-7.


Western Blot: Human
PMID Dilution Publication
28367114 1:250 Maphis, N.M., et al. 2017 Whole Genome Expression Analysis in a Mouse Model of Tauopathy Identifies MECP2 as a Possible Regulator of Tau Pathology. Frontiers in Molecular Neuroscience, 10:69.


Western Blot: Mouse
PMID Dilution Publication
28367114 1:1000 Maphis, N.M., et al. 2017 Whole Genome Expression Analysis in a Mouse Model of Tauopathy Identifies MECP2 as a Possible Regulator of Tau Pathology. Frontiers in Molecular Neuroscience, 10:69.
19225110 not listed Tao, J., et al. 2009 Phosphorylation of MeCP2 at Serine 80 regulates its chromatin association and neurological function. Proc Natl Acad Sci U S A 106(12):4882-7.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Western blot of neonatal rat brain showing specific immunolabeling of the ~75 kDa MeCP2 protein phosphorylated at Ser80.

Click here to view our Western blotting and lysate preparation protocols.

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