Affinity purified from pooled serum. Learn more.

MeCP2 (Ser421) Antibody

Catalog #: p1205-421 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Reactivity:
Mouse, Rat, Human, Non-human primate
Applications:
WB 1:1000
Species:
Rabbit
Molecular Weight:
~55 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1205-421, RRID:AB_2492145
Antigen/Purification: ExpandCollapse

The antigen is  a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser421 of MeCP2 (Methyl-CpG Binding Protein 2) protein.

The antibody is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho- peptide affinity columns.

Biological Significance: ExpandCollapse

MeCP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MeCP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A (Nan et al., 1998). Defects in MeCP2 are the cause of Rett syndrome (RTT) (Amir et al., 1999). RTT is an X-linked dominant disease; it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Recent studies have reported a new phosphorylation site at Ser421. Phosphorylation and dephosphorylation of this site may be involved in regulation of behavioral responses to chronic antidepressant treatment (Hutchinson et al., 2012).

Synonyms: ExpandCollapse

• AUTSX 3 antibody
• AUTSX3 antibody
• DKFZp686A24160 antibody
• Mbd 5 antibody
• Mbd5 antibody
• MECP 2 antibody
• MeCP 2 protein antibody
• MeCP-2 protein antibody
• Mecp2 antibody
• MECP2_HUMAN antibody
• Methyl CpG binding protein 2 (Rett syndrome) antibody
• Methyl CpG binding protein 2 antibody
• Methyl-CpG-binding protein 2 antibody
• MRX 16 antibody
• MRX 79 antibody
• MRX16 antibody
• MRX79 antibody
• MRXS 13 antibody
• MRXS13 antibody
• MRXSL antibody
• PPMX antibody
• RS antibody
• RTS antibody
• RTT antibody
• WBP 10 antibody
• WBP10 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

General References

Amir RE, Van den Veyver IB, Wan M, Tran CQ, Francke U, Zoghbi HY (1999) Rett syndrome is caused by mutations in X-linked MECP2, encoding methyl-CpG-binding protein 2. Nat Genet. Oct;23(2):185-8.

Hutchinson AN, Deng JV, Cohen S, West AE (2012) Phosphorylation of MeCP2 at Ser421 contributes to chronic antidepressant action. Journal of Neuroscience. Oct 10;32(41):14355-63.

Nan X, Ng HH, Johnson CA, Laherty CD, Turner BM, Eisenman RN, Bird A (1998) Transcriptional repression by the methyl-CpG-binding protein MeCP2 involves a histone deacetylase complex. Nature. May 28;393(6683):386-9.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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MeCP2 ser421 antibody
Western blot of mouse whole brain lysate showing specific immunolabeling of the ~55 kDa truncated MeCP2 protein phosphorylated at Ser421 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

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