Affinity purified from pooled serum. Learn more.

MeCP2 (Ser421) Antibody

We are the original manufacturer of our MeCP2 (Ser421) rabbit polyclonal phosphospecific antibody, which is affinity purified from pooled serum. Optimized in WB.

Catalog #: p1205-421 Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Mouse, Rat
Expected Reactivity:
Human, Non-human primate
Applications:
WB 1:1000 Don't see your application?
Host Species:
Rabbit Polyclonal
Molecular Weight:
~55 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1205-421, RRID:AB_2492145
Antigen/Purification: ExpandCollapse

The antigen is  a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser421 of MeCP2 (Methyl-CpG Binding Protein 2) protein.

The antibody is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho- peptide affinity columns.

Biological Significance: ExpandCollapse

MeCP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MeCP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A (Nan et al., 1998). Defects in MeCP2 are the cause of Rett syndrome (RTT) (Amir et al., 1999). RTT is an X-linked dominant disease; it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Recent studies have reported a new phosphorylation site at Ser421. Phosphorylation and dephosphorylation of this site may be involved in regulation of behavioral responses to chronic antidepressant treatment (Hutchinson et al., 2012).

Synonyms: ExpandCollapse

• AUTSX 3 antibody
• AUTSX3 antibody
• DKFZp686A24160 antibody
• Mbd 5 antibody
• Mbd5 antibody
• MECP 2 antibody
• MeCP 2 protein antibody
• MeCP-2 protein antibody
• Mecp2 antibody
• MECP2_HUMAN antibody
• Methyl CpG binding protein 2 (Rett syndrome) antibody
• Methyl CpG binding protein 2 antibody
• Methyl-CpG-binding protein 2 antibody
• MRX 16 antibody
• MRX 79 antibody
• MRX16 antibody
• MRX79 antibody
• MRXS 13 antibody
• MRXS13 antibody
• MRXSL antibody
• PPMX antibody
• RS antibody
• RTS antibody
• RTT antibody
• WBP 10 antibody
• WBP10 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Mouse


Western Blot: Mouse
PMID Dilution Publication
32138755 1:1000 Buck, J.M., et al. 2020 Developmental nicotine exposure engenders intergenerational downregulation and aberrant posttranslational modification of cardinal epigenetic factors in the frontal cortices, striata, and hippocampi of adolescent mice. Epigenetics and Chromatin, 13(1), pp.1-15.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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