mCherry Antibody

Our mCherry mouse monoclonal antibody is Protein G purified culture supernatant. Excellent fluorescence marker that does not cross react with GFP! Optimized in WB, IHC, and ICC.

Catalog #: 1203-mCherry Category: Datasheet:

$119.00$380.00

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Clear
Formulation:
Protein G purified culture supernatant
Expected Reactivity:
Specific for the ~28 kDa mCherry protein
Applications:
WB 1:1000IHC 1:500ICC 1:500 Don't see your application?
Host Species:
Mouse Monoclonal
Molecular Weight:
~ 28 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1203-mCherry, RRID:AB_2715492
Antigen/Purification: ExpandCollapse

The antigen is recombinant full length protein.

The antibody is Protein G purified from culture supernatant.

Biological Significance: ExpandCollapse

mCherry is a red fluorescent protein derived from DsRed which is isolated from disc corals and related to GFP (green fluorescent protein) (Shaner et al., 2004).  Fluorescent proteins are commonly used to tag proteins in study of their expression and in transgenic research.   Antibodies against fluorescent proteins such as mCherry are especially useful when amplification of the fluorescent signal is necessary or desired.

Synonyms: ExpandCollapse

• DSRED
• red fluorescent protein mCherry
• Red Fluoroscent Protein

Storage

100 µl in PBS + 50% glycerol and 5 mM sodium azide. Adequate amount of material to conduct 10-mini Western Blots

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunocytochemistry

HEK293 cells transfected with mCherry

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General Reference

Shaner, N. C., Campbell, R. E., Steinbach, P. A., Giepmans, B. N., Palmer, A. E., & Tsien, R. Y. (2004). Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnology, 22(12), 1567. PMID: 15558047

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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