mCherry Antibody

Catalog #: 1203-mCherry Category: Datasheet:

$109.00$365.00

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Mouse monoclonal antibody

Formulation:
Protein G purified culture supernatant
Species Reactivity:
Specific for the ~28 kDa mCherry protein
Applications:
WB 1:1000IF 1:500
Species:
Mouse Monoclonal
Molecular Reference:
~ 28 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1203-mCherry, RRID:AB_2715492
Antigen/Purification: ExpandCollapse

The antigen is recombinant full length protein.

The antibody is Protein G purified from culture supernatant.

Biological Significance: ExpandCollapse

mCherry is a red fluorescent protein derived from DsRed which is isolated from disc corals and related to GFP (green fluorescent protein) (Shaner et al., 2004).  Fluorescent proteins are commonly used to tag proteins in study of their expression and in transgenic research.   Antibodies against fluorescent proteins such as mCherry are especially useful when amplification of the fluorescent signal is necessary or desired.

Synonyms: ExpandCollapse

• DSRED
• red fluorescent protein mCherry
• Red Fluoroscent Protein

Storage

100 µl in PBS + 5 mM sodium azide.

For long term storage –20° C is recommended in undiluted aliquots. Stable at –20° C for at least 1 year. Avoid freeze/thaw cycles.

General Reference

Shaner, N. C., Campbell, R. E., Steinbach, P. A., Giepmans, B. N., Palmer, A. E., & Tsien, R. Y. (2004). Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature biotechnology, 22(12), 1567.

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Western blot of mCherry antibody
Western blot of mCherry transfected HEK293 cell lysate (+) showing specific immunolabeling of the ~28 kDa mCherry protein. Labeling is absent in the untransfected lysate (-).
IHC image of mCherry antibody
Immunofluorescence of HEK293 cells transfected with mCherry (red) and immunolabeled with mCherry antibody (green) subsequently appear yellow. Untransfected cell nuclei are stained blue

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