mCherry Antibody

Catalog #: 1203-mCherry Category: Datasheet:

$109.00$365.00

  • SizePrice
Clear

Excellent fluorescence marker that does not cross react with GFP!

Formulation:
Protein G purified culture supernatant
Species Reactivity:
Specific for the ~28 kDa mCherry protein
Applications:
WB 1:1000IHC 1:500ICC 1:500
Species:
Mouse Monoclonal
Molecular Weight:
~ 28 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1203-mCherry, RRID:AB_2715492
Antigen/Purification: ExpandCollapse

The antigen is recombinant full length protein.

The antibody is Protein G purified from culture supernatant.

Biological Significance: ExpandCollapse

mCherry is a red fluorescent protein derived from DsRed which is isolated from disc corals and related to GFP (green fluorescent protein) (Shaner et al., 2004).  Fluorescent proteins are commonly used to tag proteins in study of their expression and in transgenic research.   Antibodies against fluorescent proteins such as mCherry are especially useful when amplification of the fluorescent signal is necessary or desired.

Synonyms: ExpandCollapse

• DSRED
• red fluorescent protein mCherry
• Red Fluoroscent Protein

Storage

100 µl in PBS + 50% glycerol and 5 mM sodium azide. Adequate amount of material to conduct 10-mini Western Blots

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunocytochemistry

HEK293 cells transfected with mCherry

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General Reference

Shaner, N. C., Campbell, R. E., Steinbach, P. A., Giepmans, B. N., Palmer, A. E., & Tsien, R. Y. (2004). Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature biotechnology, 22(12), 1567. PMID: 15558047

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
Leave a Reply

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

Western blot of mCherry antibody
Western blot of mCherry transfected HEK293 cell lysate (+) showing specific immunolabeling of the ~28 kDa mCherry protein. Labeling is absent in the untransfected lysate (-).

Click here to view our Western blotting and lysate preparation protocols.
IHC image of mCherry antibody
Immunostaining of HEK293 cells transfected with mCherry(red) and stained with anti-mCherry antibody (cat #1203-mCherry, green, 1:500) subsequently appear yellow. The nuclei stained blue (DAPI) distinguishes untransfected cells.

Download the protocol used for this image:

You may also like…