Lysosomal Associated Membrane Protein 1 (LAMP1) Antibody

Catalog #: 1030-LAMP1 Categories: , , Datasheet:

$109.00$365.00

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Excellent lysosomal marker!

Formulation:
Protein G Purified
Species Tested:
Human
Applications:
WB 1:5000IHC 1:2000ICC 1:500-1:2000
Species:
Mouse
Gene Name:
LAMP1
Molecular Weight:
~100 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1030-LAMP1, RRID:AB_2492140
Antigen/Purification: ExpandCollapse

The antigen is a recombinant full length human LAMP1 expressed in and purified from E. Coli.

The antibody is Protein G purified from tissue culture supernatant.

 

Biological Significance: ExpandCollapse

Lysosomal Associated Membrane Protein1 (LAMP1) is a protein that is localized primarily in lysosomes but may also be present on late endosomes and the plasma membrane. LAMP1 antibodies are therefore widely used as lysosome markers. It has recently been suggested that lysosomes are activated in microglia in the progression of multiple system atrophy (MSA) and thus play a key role in its pathology (Makioka et al., 2012).

Synonyms: ExpandCollapse

• CD107 antigen like family member A antibody
• CD107 antigen-like family member A antibody
• CD107a antibody
• CD107a antigen antibody
• LAMP 1 antibody
• LAMP-1 antibody
• LAMP1 antibody
• LAMP1_HUMAN antibody
• LAMPA antibody
• LGP120 antibody
• lgpA antibody
• Lysosomal membrane glycoprotein 120KD antibody
• Lysosomal Associated Membrane Protein 1 antibody
• Lysosome associated membrane glycoprotein 1 antibody
• Lysosome-associated membrane glycoprotein 1 antibody
• Lysosome-associated membrane protein 1 antibody
• OTTHUMP00000040663 antibody

Storage

100 µl in PBS + 10 mM sodium azide. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Immunocytochemistry

HeLa cells

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General Reference

Makioka K, Yamazaki T, Takatama M, Nakazato Y, Okamoto K. (2012) Activation and alteration of lysosomes in multiple system atrophy. Neuroreport. Mar 28;23(5):270-6.  PMID: 22343695

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Lysosomal Associated Membrane Protein 1 (LAMP1) Antibody
Western blot of HeLa cell lysate showing specific immunolabeling of the ~100 kDa LAMP1 protein.

Click here to view our Western blotting and lysate preparation protocols.

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