Interferon-α Receptor, Type I, Subunit 1 (Ser535,539) Antibody

Catalog #: p170-5359 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Formulation:
Affinity purified
Species Tested:
Human
Species Reactivity:
Rat, Mouse, Bovine, Canine, Non-human primate, Sheep
Applications:
WB 1:1000 Don't see your application?
Species:
Rabbit
Gene Name:
IFNAR1
Molecular Weight:
~110 kDa to ~130 kDa
Cite This Antibody:
PhosphoSolutions Cat# p170-5359, RRID:AB_2492138
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser535,539 of human interferon-α receptor, type I, subunit I (IFNAR1).

The antibody is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system (Biron, 2001; Kirkwood, 2002). These cytokines produce their effects by binding to the Type 1 Interferon-α Receptor (IFNAR1). Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This down regulation is thought to be influenced by phosphorylation of Serine535 and Ser539 in the IFNAR1 (Kumar et al., 2003).

Synonyms: ExpandCollapse

• Alpha-Type Antiviral Protein
• AVP
• Beta-Type Antiviral Protein
• CRF2-1
• Cytokine Receptor Class-II Member 1
• Cytokine Receptor Family 2 Member 1
• IFN-Alpha/Beta Receptor 1
• IFNBR
• IFN-Alpha-REC
• IFNAR
• IFN-R-1
• IFRC
• Interferon Alpha/Beta Receptor 1
• Interferon-Alpha/Beta Receptor Alpha Chain
• Interferon Alpha And Beta Receptor Subunit 1
• Interferon (Alpha, Beta And Omega) Receptor 1
• Interferon-Beta Receptor 1
• Interferon Receptor 1
• Type I Interferon Receptor 1

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Human | Mouse


Western Blot: Human
PMID Dilution Publication
21865166 not listed Zheng, H., et al. 2011 Tyrosine phosphorylation of protein kinase D2 mediates ligand-inducible elimination of the Type 1 interferon receptor. Journal of Biological Chemistry, 286(41), pp.35733-35741.
21695243 not listed Qian, J., et al. 2011 Pathogen recognition receptor signaling accelerates phosphorylation-dependent degradation of IFNAR1. PLoS Pathog, 7(6), p.e1002065.
21540188 not listed Bhattacharya, S., et al. 2011 Role of p38 protein kinase in the ligand-independent ubiquitination and down-regulation of the IFNAR1 chain of type I interferon receptor. Journal of Biological Chemistry, 286(25), pp.22069-22076.
19948722 not listed Bhattacharya, S., et al. 2010 Inducible priming phosphorylation promotes ligand-independent degradation of the IFNAR1 chain of type I interferon receptor. J Biol Chem. 285(4):2318-25.
19805514 not listed Liu, J., et al. 2009 Mammalian casein kinase 1α and its leishmanial ortholog regulate stability of IFNAR1 and type I interferon signaling. Molecular and cellular biology, 29(24), pp.6401-6412.
15337770 not listed Kumar, K.G., et al. 2004 Phosphorylation and Specific Ubiquitin Acceptor Sites Are Required for Ubiquitination and Degradation of the IFNAR1 Subunit of Type I Interferon Receptor. J. Biol. Chem., Nov 2004; 279: 46614 – 46620.


Western Blot: Human
PMID Dilution Publication
21695243 not listed Qian, J., et al. 2011 Pathogen recognition receptor signaling accelerates phosphorylation-dependent degradation of IFNAR1. PLoS Pathog, 7(6), p.e1002065.
21540188 not listed Bhattacharya, S., et al. 2011 Role of p38 protein kinase in the ligand-independent ubiquitination and down-regulation of the IFNAR1 chain of type I interferon receptor. Journal of Biological Chemistry, 286(25), pp.22069-22076.
19805514 not listed Liu, J., et al. 2009 Mammalian casein kinase 1α and its leishmanial ortholog regulate stability of IFNAR1 and type I interferon signaling. Molecular and cellular biology, 29(24), pp.6401-6412.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Interferon-α Receptor, Type I, Subunit 1 ser535,539 Antibody
Western blot of immunoprecipitates from HEK 293 cells transfected with 1) Mock, 2) IFNAR1 WT, and 3) IFNAR1 S535A and S539A mutants. Specific immunolabeling of the ~110 kDa to ~130 kDa IFNAR1 WT (2) is shown in the first blot, as the immunolabeling is absent in IFNAR1 Ser535 and Ser539 mutants (3). The specific immunolabeling is blocked by the phosphopeptide (+) used as the antigen in the second blot.

Click here to view our Western blotting and lysate preparation protocols.

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