Interferon-α Receptor, Type I, Subunit 1 (Ser535,539) Antibody

Catalog #: p170-5359 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Formulation:
Affinity purified
Species Reactivity:
Rat, Mouse, Human, Bovine, Non-human primate, Sheep, Canine
Applications:
WB 1:1000IHC 1:10
Species:
Rabbit
Gene Name:
IFNAR1
Molecular Weight:
~110 kDa to ~130 kDa
Cite This Antibody:
PhosphoSolutions Cat# p170-5359, RRID:AB_2492138
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser535,539 of human interferon-? receptor, type I, subunit I (IFNAR1).

The antibody is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system (Biron, 2001; Kirkwood, 2002). These cytokines produce their effects by binding to the Type 1 Interferon-? Receptor (IFNAR1). Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This down regulation is thought to be influenced by phosphorylation of Serine 535 and 539 in the IFNAR1 (Kumar et al., 2003).

Synonyms: ExpandCollapse

• Alpha-Type Antiviral Protein
• AVP
• Beta-Type Antiviral Protein
• CRF2-1
• Cytokine Receptor Class-II Member 1
• Cytokine Receptor Family 2 Member 1
• IFN-Alpha/Beta Receptor 1
• IFNBR
• IFN-Alpha-REC
• IFNAR
• IFN-R-1
• IFRC
• Interferon Alpha/Beta Receptor 1
• Interferon-Alpha/Beta Receptor Alpha Chain
• Interferon Alpha And Beta Receptor Subunit 1
• Interferon (Alpha, Beta And Omega) Receptor 1
• Interferon-Beta Receptor 1
• Interferon Receptor 1
• Type I Interferon Receptor 1

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific References

Zheng, H., Qian, J., Baker, D.P. and Fuchs, S.Y., 2011. Tyrosine phosphorylation of protein kinase D2 mediates ligand-inducible elimination of the Type 1 interferon receptor. Journal of Biological Chemistry, 286(41), pp.35733-35741.  PMID: 21865166

Qian, J., Zheng, H., HuangFu, W.C., Liu, J., Carbone, C.J., Leu, N.A., Baker, D.P. and Fuchs, S.Y., 2011. Pathogen recognition receptor signaling accelerates phosphorylation-dependent degradation of IFNAR1. PLoS Pathog, 7(6), p.e1002065.  PMID:  21695243

Bhattacharya, S., Qian, J., Tzimas, C., Baker, D.P., Koumenis, C., Diehl, J.A. and Fuchs, S.Y., 2011. Role of p38 protein kinase in the ligand-independent ubiquitination and down-regulation of the IFNAR1 chain of type I interferon receptor. Journal of Biological Chemistry, 286(25), pp.22069-22076.  PMID:  21540188

Bhattacharya S, HuangFu WC, Liu J, Veeranki S, Baker DP, Koumenis C, Diehl JA and Fuchs SY. (2010) Inducible priming phosphorylation promotes ligand-independent degradation of the IFNAR1 chain of type I interferon receptor. J Biol Chem. 285(4):2318-25. PMID:  19948722

Liu, J., Carvalho, L.P., Bhattacharya, S., Carbone, C.J., Kumar, K.S., Leu, N.A., Yau, P.M., Donald, R.G., Weiss, M.J., Baker, D.P. and McLaughlin, K.J., 2009. Mammalian casein kinase 1? and its leishmanial ortholog regulate stability of IFNAR1 and type I interferon signaling. Molecular and cellular biology, 29(24), pp.6401-6412. PMID:  19805514

K. G. Suresh Kumar, John J. Krolewski, and Serge Y. Fuchs (2004) Phosphorylation and Specific Ubiquitin Acceptor Sites Are Required for Ubiquitination and Degradation of the IFNAR1 Subunit of Type I Interferon Receptor. J. Biol. Chem., Nov 2004; 279: 46614 – 46620. PMID: 15337770

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Interferon-α Receptor, Type I, Subunit 1 ser535,539 Antibody
Western blot of immunoprecipitates from HEK 293 cells transfected with 1) Mock, 2) IFNAR1 WT, and 3) IFNAR1 S535A and S539A mutants. Specific immunolabeling of the ~110 kDa to ~130 kDa IFNAR1 WT (2) is shown in the first blot, as the immunolabeling is absent in IFNAR1 Ser535 and Ser539 mutants (3). The specific immunolabeling is blocked by the phosphopeptide (+) used as the antigen in the second blot.

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