Affinity purified from pooled serum. Learn more.

Hsp70 (Ser153) Antibody

Catalog #: p1182-153 Category: Datasheet:

$109.00$365.00

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Rabbit Polyclonal Antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Human, wide species reactivity expected
Applications:
WB 1:1,000
Species:
Rabbit Polyclonal
Molecular Reference:
~ 70 kDA
Cite This Antibody:
PhosphoSolutions Cat# p1182-153, RRID:AB_2636998
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho Ser153 of human Hsp70.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Biological Significance: ExpandCollapse

The Hsp70 family of heat shock proteins are considered stress-induced survival proteins as they are expressed when exposed to factors such as heat, hypoxia, oxidative stress, altered pH or by underlying factors like cancer (Daugaard et al. 2007).  Hsp70 proteins are able to bind hydrophobic residues on misfolded proteins, thereby preventing their aggregation and thus serving important roles in protein homeostasis.  To date, there have been 8 members of the family identified; the majority of which are found in the cytosol though some have specific function in tissue or an organelle (Murphy, 2013).  Hsp70 has also been shown to play a major role in cancer; from tumor grade to prognosis, as well as chemotherapeutic drug resistance (Ciocca et al, 2005). There have been several phospho-serine and threonine sites identified within Hsp70, the role of each one has yet to be determined.

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

General References

Daugaard M, Rohde M, Jäättelä M (2007) The Heat Shock Protein 70 Family:  Highly homologous proteins with overlapping and distinct functions. FEBS Lett, 581: 3702-10.

Ciocca DR, Calderwood SK (2005) Heat Shock Proteins in Cancer: Diagnostic, Prognostic, Predictive, and Treatment Implications. Cell Stress Chaperones, 10: 86-103.

Murphy ME (2013) The Hsp70 Family and Cancer. Carcinogenesis, 34:1181-8.

Hsp70 ser153 Antibody
Western blot of T47D cell lysate treated with EGF (1 nM) for 60 minutes showing specific labeling of the ~70 kDa Hsp70 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is eliminated or significantly decreased with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

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