Our Anti-Hsp60 primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Hsp60 and is Protein G purified. It is great for use in WB.
Western blot of HeLa cell lysate showing specific immunolabeling of the ~60 kDa HSP60 protein.
HSP60 is a member of the heat shock protein family which is thought to play a key role in mitochondrial protein transport and proper refolding of proteins imported from the cytoplasm into the mitochondrial matrix (Koll et al., 1992). More recently, HSP60 has been implicated in the pathogenesis of cardiovascular disease (Rizzo et al., 2011).
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Protein G purified culture supernatant
PBS + 50% glycerol and 5 mM NaN3
WB: 1:1000
IHC: 1:1000-1:5000
ICC: 1:500-1:5000
Unconjugated
Specific for endogenous levels of the ~ 60 kDa HSP60 protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
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Koll, H., Guiard, B., Rassow, J., Ostermann, J., Horwich, A.L., Neupert, W. and Hartl, F.U., 1992. Antifolding activity of hsp60 couples protein import into the mitochondrial matrix with export to the intermembrane space. Cell, 68(6), pp.1163-1175. PMID: 1347713
Rizzo, M., JL Macario, A., Conway de Macario, E., Gouni-Berthold, I., K Berthold, H., Battista Rini, G., Zummo, G. and Cappello, F., 2011. Heat shock protein-60 and risk for cardiovascular disease. Current Pharmaceutical Design, 17(33), pp.3662-3668. PMID: 22074436
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