HSP60 Antibody

Catalog #: 750-HSP60 Categories: , Datasheet:

$109.00$365.00

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Mitochondrial marker that works great in IHC and ICC!

 

Formulation:
Protein G Purified
Species Tested:
Human, Mouse, Rat
Species Reactivity:
Most mammals
Applications:
WB 1:1000IHC 1:1000-1:5000 ICC 1:500-1:5000
Species:
Mouse Monoclonal
Gene Name:
HSBD1
Molecular Weight:
~ 60 kDa
Cite This Antibody:
PhosphoSolutions Cat# 750-HSP60, RRID:AB_2715493
Antigen/Purification: ExpandCollapse

The antigen is recombinant full length human HSP60 protein.

The antibody is protein G purified from culture supernatant.

Biological Significance: ExpandCollapse

HSP60 is a member of the heat shock protein family which is thought to play a key role in mitochondrial protein transport and proper refolding of proteins imported from the cytoplasm into the mitochondrial matrix (Koll et al., 1992).  More recently, HSP60 has been implicated in the pathogenesis of cardiovascular disease (Rizzo et al., 2011).

Synonyms: ExpandCollapse

• 60 kDa chaperonin antibody
• 60 kDa heat shock protein, mitochondrial antibody
• CH60_HUMAN antibody
• Chaperonin 60 antibody
• Chaperonin, 60-KD antibody
• CPN60 antibody
• fa04a05 antibody
• GROEL antibody
• heat shock 60kDa protein 1 (chaperonin) antibody
• Heat shock protein 1 (chaperonin) antibody
• Heat shock protein 60 antibody
• Heat shock protein 65 antibody
• heat shock protein family D (Hsp60) member 1 antibody
• HLD4 antibody
• Hsp 60 antibody
• HSP 65 antibody
• HSP-60 antibody
• HSP60 antibody
• HSP65 antibody
• HSPD1 antibody
• HuCHA60 antibody
• Mitochondrial matrix protein P1 antibody
• P60 lymphocyte protein antibody
• short heat shock protein 60 Hsp60s1 antibody
• SPG13 antibody

Storage

100 µl in PBS + 50% glycerol and  5 mM sodium azide. Adequate amount of material to conduct 10-mini Western Blots

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunocytochemistry

HeLa cells

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Koll H, Guiard B, Rassow J, Ostermann J, Horwich AL, Neupert W, Hartl FU. (1992)  Antifolding activity of HSP60 couples protein import into the mitochondrial matrix with export to the intermembrane space. Cell 68:1163-75.

Rizzo M, Macario AJ, de Macario EC, Gouni-Berthold I, Berthold HK, Rini GB, Zummo G, Cappello F. (2011) Heat shock protein-60 and risk for cardiovascular disease. Curr Pharm Des 17:3662-8.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Western blot of HSP60 antibody
Western blot of HeLa cell lysate showing specific immunolabeling of the ~60 kDa HSP60 protein.

Click here to view our Western blotting and lysate preparation protocols.

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