HSP60 Antibody

Our HSP60 mouse monoclonal antibody is Protein G purified. It is a mitochondrial marker that works great in WB, IHC, and ICC!

Catalog #: 750-HSP60 Categories: , , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Formulation:
Protein G Purified
Species Tested:
Human, Mouse, Rat
Expected Reactivity:
Most mammals
Applications:
WB 1:1000IHC 1:1000-1:5000 ICC 1:500-1:5000 Don't see your application?
Host Species:
Mouse Monoclonal
Gene Name:
HSBD1
Molecular Weight:
~ 60 kDa
Cite This Antibody:
PhosphoSolutions Cat# 750-HSP60, RRID:AB_2715493
Antigen/Purification: ExpandCollapse

The antigen is recombinant full length human HSP60 protein.

The antibody is protein G purified from culture supernatant.

Biological Significance: ExpandCollapse

HSP60 is a member of the heat shock protein family which is thought to play a key role in mitochondrial protein transport and proper refolding of proteins imported from the cytoplasm into the mitochondrial matrix (Koll et al., 1992).  More recently, HSP60 has been implicated in the pathogenesis of cardiovascular disease (Rizzo et al., 2011).

Synonyms: ExpandCollapse

• 60 kDa chaperonin antibody
• 60 kDa heat shock protein, mitochondrial antibody
• CH60_HUMAN antibody
• Chaperonin 60 antibody
• Chaperonin, 60-KD antibody
• CPN60 antibody
• fa04a05 antibody
• GROEL antibody
• heat shock 60kDa protein 1 (chaperonin) antibody
• Heat shock protein 1 (chaperonin) antibody
• Heat shock protein 60 antibody
• Heat shock protein 65 antibody
• heat shock protein family D (Hsp60) member 1 antibody
• HLD4 antibody
• Hsp 60 antibody
• HSP 65 antibody
• HSP-60 antibody
• HSP60 antibody
• HSP65 antibody
• HSPD1 antibody
• HuCHA60 antibody
• Mitochondrial matrix protein P1 antibody
• P60 lymphocyte protein antibody
• short heat shock protein 60 Hsp60s1 antibody
• SPG13 antibody

Storage

100 µl in PBS + 50% glycerol and  5 mM sodium azide. Adequate amount of material to conduct 10-mini Western Blots

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunocytochemistry

HeLa cells

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Koll, H., Guiard, B., Rassow, J., Ostermann, J., Horwich, A.L., Neupert, W. and Hartl, F.U., 1992. Antifolding activity of hsp60 couples protein import into the mitochondrial matrix with export to the intermembrane space. Cell, 68(6), pp.1163-1175. PMID: 1347713

Rizzo, M., JL Macario, A., Conway de Macario, E., Gouni-Berthold, I., K Berthold, H., Battista Rini, G., Zummo, G. and Cappello, F., 2011. Heat shock protein-60 and risk for cardiovascular disease. Current Pharmaceutical Design, 17(33), pp.3662-3668. PMID: 22074436

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
Leave a Reply

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

You may also like…