Affinity purified from pooled serum. Learn more.

HDAC2 (Ser394) Antibody

Catalog #: p1180-394 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Rat, Mouse, Human, Bovine, Chicken, Non-human primate, Sheep, Guinea Pig
Applications:
WB 1:1000
Species:
Rabbit
Gene Name:
HDAC2
Molecular Reference:
~55 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1180-394, RRID:AB_2492136
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser394 of human HDAC2.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Histone Deacetylase 2 (HDAC2) is part of a family of histone deacetylases that are responsible for deacetylation of lysine residues in the histone core. HDAC2 is classified as a class I histone deacetylase and is ubiquitously expressed throughout the body (Kee et al, 2008). It has been shown that HDAC2 plays an important role in cardiac hypertrophy (Eom et al, 2011). Phosphorylation of ser394 is responsible for the hypertrophy-associated activation of HDAC2, whereas intrinsic basal activity is maintained by phosphorylation of Ser422 and Ser424 (EOM et al, 2011).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

General References

Kee HJ, Eom GH, Joung H, Shin S, Kim JR Cho YK, Choe N, Sim BW, Jo D, Jeong MH, Kim KK, Seo JS, Kook H (2008) Activation of histone deacetylase 2 by inducible heat shock protein 70 in cardiac hypertrophy. Cir Res 2008 Nov 21;103(11):1259-69.

Eom GH, Cho YK, Ko JH, Shin S, Choe N, Kim Y, Joung H, Kim HS, Nam KI, Kee HJ, Kook H (2011) Casein Kinase-2α1 Induces Hypertrophic Respose by Phosphorylation of Histone Deacetylase 2 S394 and its activation in the Heart Clinical Perspective. Circulation May16, 2011;123:2392-2403.

HDAC2 ser394 Antibody
Western blot of mouse heart lysate showing specific immunolabeling of the ~55 kDa HDAC2 protein phosphorylated at Ser394 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

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