This product is produced by our partner Aves Labs. Learn more.

HA Epitope Tag Antibody

Chicken Polyclonal Antibody against the HA Epitope Tag

Catalog #: ET-HA100 Category: Datasheet:

$255.00

Size:
1 mL
Formulation:
Affinity Purified IgY
Expected Reactivity:
Specific for the HA epitope tag
Applications:
WB 1:5000IHC 1:500IP ICC ELISA
Host Species:
Chicken Polyclonal
Cite This Antibody:
Aves Labs Cat# ET-HA100, RRID:AB_2313511
Antigen/Purification: ExpandCollapse

Chickens were immunized with a synthetic peptide containing the influenza hemagglutinin (HA) epitope (i.e., YPYDVPDYA) conjugated to keyhole limpet hemocyanin. The first injection included complete Freund’s adjuvant; subsequent injections included incomplete Freund’s adju­vant. Eggs were collected after the fourth injection, and antibodies were purified from the yolks using a proprietary method that yields a purity of >90%.  Antibodies were affinity-purified over a peptide column and then dialyzed against phosphate-buffered isotonic saline. Sodium azide was added and the antibod­ies were filter-sterilized (0.2 µm).

Storage

Phosphate-buffered saline (PBS) (pH 7.2), sodium azide (0.02%, w/v) (as an anti-microbial agent).

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). For longer storage periods, dilute the antibodies with sterile glycerol and store at -20°C. Do not freeze these antibodies unless you want to store them for longer periods of time.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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