Affinity purified from pooled serum. Learn more.

GSK3β (Ser9) Antibody

Catalog #: p1175-9 Categories: , Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Rat
Expected Reactivity:
Mouse, Human, Xenopus, Bovine, Chicken, Non-human primate, Zebra fish, Canine
Applications:
WB 1:1000 Don't see your application?
Species:
Rabbit
Gene Name:
GSK3B
Molecular Weight:
~46 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1175-9, RRID:AB_2492134
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser9 of GSK3β.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Glycogen synthase kinase 3 (GSK3) is a serine/threonine kinase that is involved in the regulation of many signaling pathways. To date, 2 isoforms have been identified: GSK3α and GSK3β. Specifically, GSK3β has been shown to play a key inhibitory role in both the insulin and Wnt signaling pathways (Papkoff and Aikawa 1998). It has been suggested that Ser9 phosphorylation underlies the inhibition of GSK3β by insulin (Sutherland et al., 1993).

Synonyms: ExpandCollapse

• Glycogen Synthase Kinase 3 Beta antibody
• Glycogen synthase kinase-3 beta antibody
• GSK 3 beta antibody
• GSK-3 beta antibody
• GSK3B antibody
• GSK3B_HUMAN antibody
• GSK3beta isoform antibody
• Serine/threonine-protein kinase GSK3B antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Sutherland C, Leighton IA, Cohen P (1993) Inactivation of glycogen synthase kinase 3 beta by phosphorylation: new kinase connections in insulin and growth factor signaling. Biochem J. Nov 15;296 (Pt. 1):15-9. PMID: 8250835

Papkoff J, Aikawa M (1998) WNT-1 and HGF regulate GSK3b activity and beta catenin signaling in mammary epithelial cells. Biochem Biophys Res Commun. Jun 29;247(3):851-8. PMID: 9647782

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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